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Literature summary for 2.5.1.17 extracted from

  • Moore, T.C.; Mera, P.E.; Escalante-Semerena, J.C.
    The EutT enzyme of Salmonella enterica is a unique ATP:Cob(I)alamin adenosyltransferase metalloprotein that requires ferrous ions for maximal activity (2014), J. Bacteriol., 196, 903-910.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.17

Activating Compound

Activating Compound Comment Organism Structure
ATP the enzyme loses activity in the absence of ATP Salmonella enterica

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Salmonella enterica

Protein Variants

Protein Variants Comment Organism
C79A the mutant shows 1 order of magnitude reduced catalytic efficiency compared to the wild type enzyme Salmonella enterica
C80A inactive Salmonella enterica
C83A the mutant shows 30fold reduced catalytic efficiency compared to the wild type enzyme Salmonella enterica
H67A the mutant shows 100fold reduced catalytic efficiency compared to the wild type enzyme Salmonella enterica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 cob(I)alamin at pH 7.0 and 37°C Salmonella enterica

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required for maximum activity Salmonella enterica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 2 * 30000, SDS-PAGE Salmonella enterica

Organism

Organism UniProt Comment Textmining
Salmonella enterica
-
-
-
Salmonella enterica JE6583
-
-
-

Oxidation Stability

Oxidation Stability Organism
the enzyme loses all activity upon exposure to air and H2O2 Salmonella enterica

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Sephadex G-25 gel filtration Salmonella enterica

Storage Stability

Storage Stability Organism
-20°C, in 50% glycerol, 6 months, no loss of activity Salmonella enterica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + cob(I)alamin
-
 Salmonella enterica triphosphate + adenosylcob(I)alamine
-
 ?
ATP + cob(I)alamin
-
 Salmonella enterica JE6583 triphosphate + adenosylcob(I)alamine
-
 ?
ATP + cob(II)alamin
-
 Salmonella enterica triphosphate + adenosylcob(II)alamine
-
 ?
ATP + cob(II)alamin
-
 Salmonella enterica JE6583 triphosphate + adenosylcob(II)alamine
-
 ?
additional information the enzyme can use free dihydroflavins to drive the adenosylation of cob(II)alamin Salmonella enterica ?
-
 ?
additional information the enzyme can use free dihydroflavins to drive the adenosylation of cob(II)alamin Salmonella enterica JE6583 ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 30000, SDS-PAGE Salmonella enterica

Synonyms

Synonyms Comment Organism
ATP:cob(I)alamin adenosyltransferase
-
 Salmonella enterica
EutT
-
 Salmonella enterica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.06
-
 cob(I)alamin at pH 7.0 and 37°C Salmonella enterica