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Literature summary for 2.5.1.17 extracted from

  • Pallares, I.G.; Moore, T.C.; Escalante-Semerena, J.C.; Brunold, T.C.
    Spectroscopic studies of the Salmonella enterica adenosyltransferase enzyme SeCobA: molecular-level insight into the mechanism of substrate Cob(II)alamin activation (2014), Biochemistry, 53, 7969-7982.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.17

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Salmonella enterica

Protein Variants

Protein Variants Comment Organism
F91W with cob(II)alamin the mutant shows catalytic activity diminished relative to that of wild type enzyme, while the activity with cob(I)alamin is largely retained Salmonella enterica
F91W/W93F in this mutant the yield of 4c cob(II)alamin species is reduced more than 10fold from that achieved by the wild type enzyme Salmonella enterica
F91Y with cob(II)alamin the mutant shows catalytic activity 5fold increased relative to that of wild type enzyme Salmonella enterica
W93A the mutation completely abolishes the catalytic activity with cob(II)alamin while modest activity is retained with cob(I)alamin Salmonella enterica
W93F the mutant shows wild type activity Salmonella enterica
W93H the mutation inhibits the formation of 4c cob(II)alamin Salmonella enterica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0163
-
 cob(II)inamide wild type enzyme, pH and temperature not specified in the publication Salmonella enterica
0.0254
-
 ATP wild type enzyme, pH and temperature not specified in the publication Salmonella enterica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + cob(I)yrinic acid a,c-diamide Salmonella enterica
-
 triphosphate + adenosylcob(III)yrinic acid a,c-diamide
-
 ?
ATP + cobinamide Salmonella enterica
-
 triphosphate + adenosylcobinamide
-
 ?

Organism

Organism UniProt Comment Textmining
Salmonella enterica P31570 serovar typhimurium LT2
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap nickel affinity column chromatography Salmonella enterica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + cob(I)alamine
-
 Salmonella enterica triphosphate + adenosylcob(I)alamine
-
 ?
ATP + cob(I)yrinic acid a,c-diamide
-
 Salmonella enterica triphosphate + adenosylcob(III)yrinic acid a,c-diamide
-
 ?
ATP + cob(II)alamine
-
 Salmonella enterica triphosphate + adenosylcob(II)alamine
-
 ?
ATP + cob(II)inamide
-
 Salmonella enterica triphosphate + adenosylcob(II)inamide
-
 ?
ATP + cobinamide
-
 Salmonella enterica triphosphate + adenosylcobinamide
-
 ?

Synonyms

Synonyms Comment Organism
ACAT
-
 Salmonella enterica
ATP:co(I)rrinoid adenosyltransferase
-
 Salmonella enterica
CobA
-
 Salmonella enterica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0067
-
 ATP wild type enzyme, pH and temperature not specified in the publication Salmonella enterica
0.0077
-
 cob(II)inamide wild type enzyme, pH and temperature not specified in the publication Salmonella enterica

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.26
-
 ATP wild type enzyme, pH and temperature not specified in the publication Salmonella enterica
0.47
-
 cob(II)inamide wild type enzyme, pH and temperature not specified in the publication Salmonella enterica