Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
R186W | catalytically inactive patient mutation leading to the inherited disorder methylmalonic aciduria. Mutant is examined using intrinsic fluorescence quenching of MMAB as a measure of ligand-binding. R190H and R186W significantly disrupt the affinity between MMAB and adenosylcobalmin. Arg 186 and Arg-190 may be critical for the transfer of the 5'-deoxyadenosyl group from ATP to cob(I)alamin, possibly by contributing to the precise positioning of the two substrates to permit catalysis to occur | Homo sapiens |
R190H | catalytically inactive patient mutation leading to the inherited disorder methylmalonic aciduria. Mutant is examined using intrinsic fluorescence quenching of MMAB as a measure of ligand-binding. R190H and R186W significantly disrupt the affinity between MMAB and adenosylcobalmin. Arg 186 and Arg-190 may be critical for the transfer of the 5'-deoxyadenosyl group from ATP to cob(I)alamin, possibly by contributing to the precise positioning of the two substrates to permit catalysis to occur | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using a HiTrap SP HP FPLC column and gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cob(I)alamin + ATP | dissociation constant (Kd) of wild-type MMAB for hydroxomethylcobalamin is 0.051 mM and for ATP is 0.365 mM, cobalamin enhances the affinity of MMAB for ATP, while ATP does not show detectable effects on cobalamin binding | Homo sapiens | adenosylcobalamin + triphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
methylmalonic aciduria type B | - |
Homo sapiens |
MMAB | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |