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Literature summary for 2.5.1.17 extracted from

  • Zhang, J.; Wu, X.; Padovani, D.; Schubert, H.; Gravel, R.
    Ligand-binding by catalytically inactive mutants of the cblB complementation group defective in human ATP:cob(I)alamin adenosyltransferase (2009), Mol. Genet. Metab., 98, 278-284.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
R186W catalytically inactive patient mutation leading to the inherited disorder methylmalonic aciduria. Mutant is examined using intrinsic fluorescence quenching of MMAB as a measure of ligand-binding. R190H and R186W significantly disrupt the affinity between MMAB and adenosylcobalmin. Arg 186 and Arg-190 may be critical for the transfer of the 5'-deoxyadenosyl group from ATP to cob(I)alamin, possibly by contributing to the precise positioning of the two substrates to permit catalysis to occur Homo sapiens
R190H catalytically inactive patient mutation leading to the inherited disorder methylmalonic aciduria. Mutant is examined using intrinsic fluorescence quenching of MMAB as a measure of ligand-binding. R190H and R186W significantly disrupt the affinity between MMAB and adenosylcobalmin. Arg 186 and Arg-190 may be critical for the transfer of the 5'-deoxyadenosyl group from ATP to cob(I)alamin, possibly by contributing to the precise positioning of the two substrates to permit catalysis to occur Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
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-
-

Purification (Commentary)

Purification (Comment) Organism
using a HiTrap SP HP FPLC column and gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cob(I)alamin + ATP dissociation constant (Kd) of wild-type MMAB for hydroxomethylcobalamin is 0.051 mM and for ATP is 0.365 mM, cobalamin enhances the affinity of MMAB for ATP, while ATP does not show detectable effects on cobalamin binding Homo sapiens adenosylcobalamin + triphosphate
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Synonyms

Synonyms Comment Organism
methylmalonic aciduria type B
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Homo sapiens
MMAB
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Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
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assay at Homo sapiens