Cloned (Comment) | Organism |
---|---|
from genomic DNA in pET-28a for expression in Escherichia coli BL21 (DE3) | Bacillus cereus |
Crystallization (Comment) | Organism |
---|---|
apoenzyme and in complex with Mg2+/ATP, C-centred orthorhombic space group C222(1), unit cell parameters: a: 64.93 A, b: 137.08 A, c: 158.55 A, alpha, beta, gamma: 90°, one trimer in the asymmetric unit, sitting-drop combined with hanging-drop vapour-diffusion method: 13 mg/ml protein solution, precipitants: 1.55-1.6 M ammonium sulfate, 9-10% (v/v) dioxane (pH 6.5), for complex: 4 mM ATP and 4 mM Mg2+ | Bacillus cereus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nickel-chelating HiTrap chromatography (elution with 1 M imidazole), HiLoad 16/60 Superdex 200 gel filtration chromatography (2 mM dithiothreitol), concentration to 13 mg/ml by ultracentrifugation | Bacillus cereus |
Synonyms | Comment | Organism |
---|---|---|
PduO | - |
Bacillus cereus |
PduO enzyme | - |
Bacillus cereus |
PduO-type ATP:cob(I)alamin adenosyltransferase | - |
Bacillus cereus |