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Literature summary for 2.5.1.17 extracted from

  • Buan, N.R.; Rehfeld, K.; Escalante-Semerena, J.C.
    Studies of the CobA-type ATP:Co(I)rrinoid adenosyltransferase enzyme of Methanosarcina mazei strain Go1 (2006), J. Bacteriol., 188, 3543-3550.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.17

Cloned(Commentary)

Cloned (Comment) Organism
gene cobAMm, expression in a Salmonella enterica strain lacking the housekeeping CobA enzyme restores coenzyme B12 synthesis, overexpression in Escherichia coli strain BL21(DE3) Methanosarcina mazei

Protein Variants

Protein Variants Comment Organism
additional information enzyme expression restores coenzyme B12 synthesis in a Salmonella enterica strain lacking the housekeeping CobA enzyme Methanosarcina mazei

Inhibitors

Inhibitors Comment Organism Structure
diphosphate 28% inhibition Methanosarcina mazei
Mn2+ 50% inhibition Methanosarcina mazei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 cob(I)alamin pH 8.0, 37°C Methanosarcina mazei
0.003
-
 ATP pH 8.0, 37°C Methanosarcina mazei

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Methanosarcina mazei 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates 2.5fold Methanosarcina mazei
Co2+ 1.75fold activation Methanosarcina mazei
additional information no activation by Na+ and Mg2+ Methanosarcina mazei
Ni2+ 1.5fold activation Methanosarcina mazei
Zn2+ 1.25fold activation Methanosarcina mazei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cob(I)alamin + ATP Methanosarcina mazei
-
 adenosylcobalamin + triphosphate
-
 ?
cob(I)inamide + ATP Methanosarcina mazei
-
 adenosylcobinamide + triphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Methanosarcina mazei
-
 orf MM3138, housekeeping gene cobAMm
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme by anion exchange chromatography, dialysis, and hydrophobic interaction chromatography, recombinant enzyme from Escherichia coli strain BL21(DE3) Methanosarcina mazei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.001
-
 purified enzyme, pH 8.0 Methanosarcina mazei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cob(I)alamin + ADP
-
 Methanosarcina mazei adenosylcobalamin + diphosphate
-
 ?
cob(I)alamin + ATP
-
 Methanosarcina mazei adenosylcobalamin + triphosphate
-
 ?
cob(I)alamin + ATP cobalamin is a better substrate than cobinamide, the beta-phosphate of ATP is required for binding to the enzyme Methanosarcina mazei adenosylcobalamin + triphosphate
-
 ?
cob(I)inamide + ATP
-
 Methanosarcina mazei adenosylcobinamide + triphosphate
-
 ?
cob(I)inamide + ATP cobalamin is a better substrate than cobinamide, the beta-phosphate of ATP is required for binding to the enzyme Methanosarcina mazei adenosylcobinamide + triphosphate
-
 ?

Synonyms

Synonyms Comment Organism
CobA-type ATP:Co(I)rrinoid adenosyltransferase
-
 Methanosarcina mazei
CobAMm
-
 Methanosarcina mazei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Methanosarcina mazei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Methanosarcina mazei

pH Range

pH Minimum pH Maximum Comment Organism
7.5 8.5 75% of maximal activity at pH 7.5, 94% at pH 8.5 Methanosarcina mazei

Cofactor

Cofactor Comment Organism Structure
ADP
-
 Methanosarcina mazei
ATP the beta-phosphate of ATP is required for binding to the enzyme Methanosarcina mazei