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Literature summary for 2.5.1.17 extracted from

  • Vitols, E.; Walker, G.A.; Huennekens, F.M.
    Enzymatic conversion of vitamin B-12s to a cobamide coenzyme, alpha-(5,6-dimethylbenzimidazolyl)deoxyadenosylcobamide (adenosyl-B-12) (1966), J. Biol. Chem., 241, 1455-1461.
    View publication on PubMed
Search for more literature for 2.5.1.17

General Stability

General Stability Organism
2-mercaptoethanol stabilizes during purification Clostridium tetanomorphum
repeated freeze-thawing inactivates Clostridium tetanomorphum

Inhibitors

Inhibitors Comment Organism Structure
diphosphate
-
 Clostridium tetanomorphum
EDTA
-
 Clostridium tetanomorphum
additional information no inhibition by phosphate Clostridium tetanomorphum
Trimetaphosphate
-
 Clostridium tetanomorphum
tripolyphosphate
-
 Clostridium tetanomorphum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 cyanocob(I)alamin pH 8.0, 37°C, i.e. vitamin B12, reduced form Clostridium tetanomorphum
0.016
-
 ATP pH 8.0, 37°C Clostridium tetanomorphum

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Clostridium tetanomorphum
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activation, less effective than Mn2+ Clostridium tetanomorphum
Mg2+ activation, less effective than Mn2+ Clostridium tetanomorphum
Mn2+
-
 Clostridium tetanomorphum
additional information no activation by Ca2+ or Cd2+ Clostridium tetanomorphum
Zn2+ activation, less effective than Mn2+ Clostridium tetanomorphum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + cob(I)alamin Clostridium tetanomorphum involved in vitamin B12 metabolism triphosphate + coenzyme B12
-
 ?

Organism

Organism UniProt Comment Textmining
Clostridium tetanomorphum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
protamine sulfate, heat treatment and chromatography on Sephadex G-25 and DEAE-cellulose Clostridium tetanomorphum

Storage Stability

Storage Stability Organism
-20°C, lyophilized, at least 1 week Clostridium tetanomorphum
2-mercaptoethanol stabilizes during storage Clostridium tetanomorphum
4°C, about 1 month Clostridium tetanomorphum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + cob(I)alamin transfers the adenosyl-group of ATP to the reduced cobalt atom of the cobalamin molecule Clostridium tetanomorphum triphosphate + coenzyme B12 adenosylcobalamin, B12-coenzyme or deoxyadenosyl-B12 ?
ATP + cob(I)alamin involved in vitamin B12 metabolism Clostridium tetanomorphum triphosphate + coenzyme B12
-
 ?
cyanocob(I)alamin + ATP
-
 Clostridium tetanomorphum tripolyphosphate + alpha-(5,6-dimethylbenzimidazolyl)deoxyadenosylcobamide
-
 ?
additional information S-adenosylmethionine, vitamin B12r or B12a, AMP, ADP are no substrates Clostridium tetanomorphum ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Clostridium tetanomorphum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 no appreciable loss of activity after 10 min Clostridium tetanomorphum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 phosphate or Tris buffer Clostridium tetanomorphum