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Literature summary for 2.5.1.16 extracted from

  • Amano, Y.; Namatame, I.; Tateishi, Y.; Honboh, K.; Tanabe, E.; Niimi, T.; Sakashita, H.
    Structural insights into the novel inhibition mechanism of Trypanosoma cruzi spermidine synthase (2015), Acta Crystallogr. Sect. D, 71, 1879-1889.
    View publication on PubMed
Search for more literature for 2.5.1.16

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Trypanosoma cruzi

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with decarboxylated S-adenosylmethionine, trans-4-methylcyclohexylamine and inhibitors, sitting drop vapor diffusion method, using 100 mM bis-Tris pH 5.5-6.5, 200 mM ammonium sulfate, 10-15% (w/v) PEG 4000 Trypanosoma cruzi

Inhibitors

Inhibitors Comment Organism Structure
2-phenyl-1,2-thiazol-3(2H)-one
-
 Trypanosoma cruzi
2H-1,4-benzothiazin-3-amine
-
 Trypanosoma cruzi
5-[(4-methylbenzyl)oxy]quinazolin-2-amine
-
 Trypanosoma cruzi
isoquinolin-1-amine
-
 Trypanosoma cruzi
N-methyl-N-phenyl-2,3-dihydro-1,4-benzodioxine-2-carboxamide
-
 Trypanosoma cruzi
quinolin-8-yl piperidine-1-carboxylate
-
 Trypanosoma cruzi
S-adenosyl-1,8-diamino-3-thio-octane
-
 Trypanosoma cruzi
trans-4-Methylcyclohexylamine
-
 Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl 3-(methylthio)propylamine + putrescine Trypanosoma cruzi
-
 S-methyl-5'-thioadenosine + spermidine
-
 ?

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi Q4DA73
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 75 gel filtration Trypanosoma cruzi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl 3-(methylthio)propylamine + putrescine
-
 Trypanosoma cruzi S-methyl-5'-thioadenosine + spermidine
-
 ?

Subunits

Subunits Comment Organism
homodimer
-
 Trypanosoma cruzi

Synonyms

Synonyms Comment Organism
SPDSYN
-
 Trypanosoma cruzi

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000051
-
 pH and temperature not specified in the publication Trypanosoma cruzi 2-phenyl-1,2-thiazol-3(2H)-one
0.0091
-
 pH and temperature not specified in the publication Trypanosoma cruzi 5-[(4-methylbenzyl)oxy]quinazolin-2-amine
0.18
-
 pH and temperature not specified in the publication Trypanosoma cruzi 2H-1,4-benzothiazin-3-amine
0.46
-
 pH and temperature not specified in the publication Trypanosoma cruzi isoquinolin-1-amine
0.53
-
 pH and temperature not specified in the publication Trypanosoma cruzi N-methyl-N-phenyl-2,3-dihydro-1,4-benzodioxine-2-carboxamide
0.83
-
 pH and temperature not specified in the publication Trypanosoma cruzi quinolin-8-yl piperidine-1-carboxylate