Cloned (Comment) | Organism |
---|---|
recombinant overexpression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain SG13009 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C159A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Escherichia coli |
D158A | site-directed mutagenesis, inactive mutant | Escherichia coli |
D161A | site-directed mutagenesis, inactive mutant | Escherichia coli |
I163A | site-directed mutagenesis, replacement of Ile163 has no influence on EcSDPS activity | Escherichia coli |
P162A | site-directed mutagenesis, replacement of Pro162 has no influence on EcSDPS activity | Escherichia coli |
P165Q | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Escherichia coli |
T160A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant SPS mutant I163A | Escherichia coli | |
0.032 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant wild-type enzyme | Escherichia coli | |
0.033 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant SPS mutant C159S | Escherichia coli | |
0.035 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant SPS mutant P162A | Escherichia coli | |
0.038 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant SPS mutant C159A | Escherichia coli | |
0.05 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant SPS mutant T160A | Escherichia coli | |
0.059 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant SPS mutant P165A | Escherichia coli | |
0.0778 | - |
putrescine | pH 7.5, 37°C, recombinant wild-type enzyme | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33252 | - |
2 * 36500, 2 * 33823, recombinant His6-tagged enzyme, mass spectrometry, 2 * 33252, His6-tagged enzyme, sequence calculation | Escherichia coli |
33823 | - |
2 * 36500, 2 * 33823, recombinant His6-tagged enzyme, mass spectrometry, 2 * 33252, His6-tagged enzyme, sequence calculation | Escherichia coli |
36500 | - |
2 * 36500, 2 * 33823, recombinant His6-tagged enzyme, mass spectrometry, 2 * 33252, His6-tagged enzyme, sequence calculation | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosylmethioninamine + putrescine | Escherichia coli | - |
S-methyl-5'-thioadenosine + spermidine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P09158 | strain MG1655, gene speE | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain SG13009 | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl 3-(methylsulfanyl)propylamine + putrescine = S-methyl-5'-thioadenosine + spermidine | Escherichia coli SPDS follows the ping-pong mechanism, in which an aminopropylated enzyme intermediate was formed by the interaction of SPDS with dcSAM, prior to reaction with putrescine | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosylmethioninamine + putrescine | - |
Escherichia coli | S-methyl-5'-thioadenosine + spermidine | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 36500, 2 * 33823, recombinant His6-tagged enzyme, mass spectrometry, 2 * 33252, His6-tagged enzyme, sequence calculation | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
SPDS | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Escherichia coli |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 50 | sharp drop in activity above optimal temperature 50°C, profile overview | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.128 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant SPS mutant C159A | Escherichia coli | |
0.135 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant SPS mutant T160A | Escherichia coli | |
0.138 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant SPS mutant I163A | Escherichia coli | |
0.14 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant wild-type enzyme | Escherichia coli | |
0.145 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant SPS mutant C159S | Escherichia coli | |
0.15 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant SPS mutant P165A | Escherichia coli | |
0.156 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant SPS mutant P162A | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | spermidine synthase plays a crucial role in cell proliferation and differentiation | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.5 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant SPS mutant P165A | Escherichia coli | |
2.7 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant SPS mutant T160A | Escherichia coli | |
3.4 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant SPS mutant C159A | Escherichia coli | |
4.4 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant wild-type enzyme and SPS mutant C159S | Escherichia coli | |
4.5 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant SPS mutant P162A | Escherichia coli | |
4.8 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant SPS mutant I163A | Escherichia coli |