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Literature summary for 2.5.1.16 extracted from

  • Taylor, M.C.; Kaur, H.; Blessington, B.; Kelly, J.M.; Wilkinson, S.R.
    Validation of spermidine synthase as a drug target in African trypanosomes (2008), Biochem. J., 409, 563-569.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Trypanosoma brucei

Protein Variants

Protein Variants Comment Organism
additional information downregulation by RNAi correlates with a decrease in intracellular spermidine and cessation of growth in bloodstream form parasites. Phenotype can be rescued by expression of Leishmania major spermidine synthase gene, but not by addition of spermidine to the growth medium Trypanosoma brucei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00009
-
S-adenosylmethioninamine pH 7.2, 37°C Trypanosoma brucei
0.205
-
putrescine pH 7.2, 37°C Trypanosoma brucei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
x * 33000, calculated, x * 37000, SDS-PAGE Trypanosoma brucei
37000
-
x * 33000, calculated, x * 37000, SDS-PAGE Trypanosoma brucei

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei Q38EH6
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Trypanosoma brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
putrescine + S-adenosylmethioninamine
-
Trypanosoma brucei spermidine + 5'-methylthioadenosine
-
?

Subunits

Subunits Comment Organism
? x * 33000, calculated, x * 37000, SDS-PAGE Trypanosoma brucei

Synonyms

Synonyms Comment Organism
SpSyn
-
Trypanosoma brucei