Application | Comment | Organism |
---|---|---|
drug development | the enzyme is a drug target in the malaria parasite, Plasmodium falciparum, due to the vital role of spermidine in the activation of the eukaryotic translation initiation factor and cell proliferation | Plasmodium falciparum |
Crystallization (Comment) | Organism |
---|---|
construction of a crystal structure homology model, PDB ID: Q9FS5, for the Plasmodium falciparum enzyme from crystal structure 1JQ3 and 1XJ5, structure-function relationship | Plasmodium falciparum |
Protein Variants | Comment | Organism |
---|---|---|
D196N | site-directed mutagenesis, the mutant activity is reduced by 89% compared to the wild-type enzyme | Plasmodium falciparum |
S197A | site-directed mutagenesis, the mutant activity is reduced by 24% compared to the wild-type enzyme | Plasmodium falciparum |
Y102A | site-directed mutagenesis, the mutant activity is reduced by 91% compared to the wild-type enzyme | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
trans-4-Methylcyclohexylamine | i.e. 4MCHA, binding structure | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | molecular dynamics | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosylmethioninamine + putrescine | Plasmodium falciparum | spermidine plays an important role in the activation of the eukaryotic translation initiation factor and cell proliferation | 5'-S-methyl-5'-thioadenosine + spermidine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl 3-(methylsulfanyl)propylamine + putrescine = S-methyl-5'-thioadenosine + spermidine | reaction mechanism involving a gate-keeping loop, active site and substrate binding structures and involved residues, structure-function relationship analysis by molecular dynamics simulations of a homology model | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosylmethioninamine + putrescine | - |
Plasmodium falciparum | 5'-S-methyl-5'-thioadenosine + spermidine | - |
? | |
S-adenosylmethioninamine + putrescine | spermidine plays an important role in the activation of the eukaryotic translation initiation factor and cell proliferation | Plasmodium falciparum | 5'-S-methyl-5'-thioadenosine + spermidine | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structure-function relationship, overview | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
SPDSYN | - |
Plasmodium falciparum |