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Literature summary for 2.5.1.16 extracted from

  • Yoon, S.O.; Lee, Y.S.; Lee, S.H.; Cho, Y.D.
    Polyamine synthesis in plants: isolation and characterization of spermidine synthase from soybean (Glycine max) axes (2000), Biochim. Biophys. Acta, 1475, 17-26.
    View publication on PubMed

General Stability

General Stability Organism
freeze-thawing inactivates Glycine max
S-adenosyl-(5')-3-methylthio-1-propylamine protects from inhibitors when preincubated Glycine max

Inhibitors

Inhibitors Comment Organism Structure
DTNB potent inhibition, activity is restored to more than 95% with 0.1 mM DTT Glycine max
N-ethylmaleimide potent inhibition Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0004
-
S-adenosyl-(5')-3-methylthio-1-propylamine pH 8.0, 37ºC Glycine max
0.0325
-
1,4-diaminobutane pH 8.0, 37ºC Glycine max

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Glycine max 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 0.01 mM, activity increases by 200% Glycine max
Co2+ 0.01 mM, activity increases by 352% Glycine max
Cu2+ 0.01 mM, activity increases by 230% Glycine max
K+ not effective Glycine max
additional information Li+, Mn2+ and Zn2+, not effective Glycine max
Na+ not effective Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74000
-
gel filtration and SDS-PAGE Glycine max

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane Glycine max
-
5'-methylthioadenosine + spermidine
-
?

Organism

Organism UniProt Comment Textmining
Glycine max
-
soybean
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation, chromatography on DEAE-Sephacel, chromatography on Sephacryl S-300, chromatography on aminooctyl-Sepharose and ATPA-Sepharose, 319fold purified Glycine max

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl 3-(methylsulfanyl)propylamine + putrescine = S-methyl-5'-thioadenosine + spermidine uni uni uni uni ping pong mechanism Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
stem 3 days old Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.076
-
-
Glycine max

Storage Stability

Storage Stability Organism
0-4ºC, 10% loss of activity after several months Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane
-
Glycine max 5'-methylthioadenosine + spermidine + H+ ?
S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane
-
Glycine max 5'-methylthioadenosine + spermidine
-
?

Subunits

Subunits Comment Organism
monomer 1 * 74000, gel filtration and SDS-PAGE Glycine max

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
stable over 30 min Glycine max
37
-
stable over 30 min Glycine max
50
-
95% loss of activity over 30 min Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
activity is 20% higher in Tris-HCl buffer than in phosphate buffer at the same pH Glycine max