General Stability | Organism |
---|---|
freeze-thawing inactivates | Glycine max |
S-adenosyl-(5')-3-methylthio-1-propylamine protects from inhibitors when preincubated | Glycine max |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DTNB | potent inhibition, activity is restored to more than 95% with 0.1 mM DTT | Glycine max | |
N-ethylmaleimide | potent inhibition | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
S-adenosyl-(5')-3-methylthio-1-propylamine | pH 8.0, 37ºC | Glycine max | |
0.0325 | - |
1,4-diaminobutane | pH 8.0, 37ºC | Glycine max |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Glycine max | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 0.01 mM, activity increases by 200% | Glycine max | |
Co2+ | 0.01 mM, activity increases by 352% | Glycine max | |
Cu2+ | 0.01 mM, activity increases by 230% | Glycine max | |
K+ | not effective | Glycine max | |
additional information | Li+, Mn2+ and Zn2+, not effective | Glycine max | |
Na+ | not effective | Glycine max |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
74000 | - |
gel filtration and SDS-PAGE | Glycine max |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane | Glycine max | - |
5'-methylthioadenosine + spermidine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
soybean | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation, chromatography on DEAE-Sephacel, chromatography on Sephacryl S-300, chromatography on aminooctyl-Sepharose and ATPA-Sepharose, 319fold purified | Glycine max |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl 3-(methylsulfanyl)propylamine + putrescine = S-methyl-5'-thioadenosine + spermidine | uni uni uni uni ping pong mechanism | Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
stem | 3 days old | Glycine max | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.076 | - |
- |
Glycine max |
Storage Stability | Organism |
---|---|
0-4ºC, 10% loss of activity after several months | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane | - |
Glycine max | 5'-methylthioadenosine + spermidine | + H+ | ? | |
S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane | - |
Glycine max | 5'-methylthioadenosine + spermidine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 74000, gel filtration and SDS-PAGE | Glycine max |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
stable over 30 min | Glycine max |
37 | - |
stable over 30 min | Glycine max |
50 | - |
95% loss of activity over 30 min | Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
activity is 20% higher in Tris-HCl buffer than in phosphate buffer at the same pH | Glycine max |