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Literature summary for 2.5.1.16 extracted from

  • Graser, G.; Hartmann T.
    Biosynthesis of spermidine, a direct precursor of pyrrolizidine alkaloids in root cultures of Senecio vulgaris L. (2000), Planta, 211, 239-245.
    View publication on PubMed
Search for more literature for 2.5.1.16

Inhibitors

Inhibitors Comment Organism Structure
1,3-diaminopropane 96% inhibition at 0.01 mM and 100% inhibition at 0.1 mM Senecio vulgaris
5'-methylthioadenosine 83% inhibition at 0.01 mM and 22% inhibition at 1 mM Senecio vulgaris
agmatine 89% inhibition at 0.1 mM and 61% inhibition at 1 mM Senecio vulgaris
arginine 98% inhibition at 0.01 mM, 95% inhibition at 0.1 mM and 84% inhibition at 1 mM Senecio vulgaris
cadaverine 77% inhibition at 0.01 mM and 22% inhibition at 1 mM Senecio vulgaris
Cyclohexylamine
-
 Senecio vulgaris
Homospermidine 95% inhibition at 0.01 mM, 92% inhibition at 0.1 mM and 30% inhibition at 1 mM Senecio vulgaris
S-adenosyl-(5')-3-methylthio-1-propylamine complete inhibition at 0.080 mM Senecio vulgaris
spermidine product inhibition at physiological concentrations Senecio vulgaris
spermine 99% inhibition at 0.1 mM and 90% inhibition at 1 mM Senecio vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 S-adenosyl-(5')-3-methylthio-1-propylamine pH 7.5, 34ºC Senecio vulgaris
0.021
-
 1,4-diaminobutane pH 7.5, 34ºC Senecio vulgaris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 gel filtration Senecio vulgaris

Organism

Organism UniProt Comment Textmining
Senecio vulgaris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, streptomycin sulfate precipitation, ammonium sulfate fractionation and gel filtration, 18fold purified Senecio vulgaris

Source Tissue

Source Tissue Comment Organism Textmining
root 10 days old Senecio vulgaris
-

Storage Stability

Storage Stability Organism
-20ºC, partially purified enzyme, 0.1 M NaCl and 1% bovine serum albumin, loss of 50% of activity after 15 days Senecio vulgaris
4ºC, crude extracts, loss of activity within 24 hours Senecio vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane
-
 Senecio vulgaris 5'-methylthioadenosine + spermidine
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
34
-
 assay at Senecio vulgaris

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 above, progressive inactivation Senecio vulgaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
 in Gly-NaOH buffer Senecio vulgaris

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
 half-maximal activities at pH 7.2 and 8.4 Senecio vulgaris