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Literature summary for 2.5.1.16 extracted from
- Purification of spermidine synthase from rat ventral prostate by affinity chromatography on immobilized S-adenosyl(5)-3-thiopropylamine (1982), Arch. Biochem. Biophys., 216, 213-222.
General Stability
| General Stability | Organism |
|---|---|
| DTT stabilizes | Rattus norvegicus |
| freeze-thawing inactivates | Escherichia coli |
| freeze-thawing inactivates | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,5-Diaminopentane | weak, competitive | Rattus norvegicus |  |
| 5'-deoxy-5'-methylthioadenosine | above 0.05 mM | Rattus norvegicus | |
| 5'-deoxy-5'-methylthiotubercidine | - |
Rattus norvegicus | |
| additional information | no inhibition by 1,4-diaminobutane | Escherichia coli | |
| additional information | no inhibition by putrescine up to 10 mM | Rattus norvegicus | |
| N-ethylmaleimide | - |
Escherichia coli | |
| N-ethylmaleimide | - |
Rattus norvegicus | |
| PCMB | - |
Rattus norvegicus | |
| S-adenosyl-(5')-3-methylthio-1-propylamine | competitive substrate inhibition | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
1,4-diaminobutane | pH 7.2, 37ºC | Rattus norvegicus | |
| 1.1 | - |
S-adenosyl-(5')-3-methylthio-1-propylamine | pH 7.2, 37ºC | Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | 10 mM, activity increases by 20% | Rattus norvegicus | |
| Mg2+ | 10 mM, activity increases 20% | Rattus norvegicus | |
| Na+ | 10 mM, activity increases by 20% | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
2 * 37000, SDS-PAGE | Rattus norvegicus |
| 73000 | - |
- |
Escherichia coli |
| 73000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane | Escherichia coli | - |
5'-methylthioadenosine + spermidine | - |
? | |
| S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane | Rattus norvegicus | - |
5'-methylthioadenosine + spermidine | - |
? | |
| S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane | Rattus norvegicus Wistar | - |
5'-methylthioadenosine + spermidine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Rattus norvegicus | - |
Wistar strain | - |
| Rattus norvegicus Wistar | - |
Wistar strain | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity chromatography on S-adenosyl(5')-3-thiopropylamine-Sepharose | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| prostate gland | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.3 | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0ºC, 30% loss of activity after 4 months | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane | - |
Escherichia coli | 5'-methylthioadenosine + spermidine | - |
? | |
| S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane | linear at least up to 6 nmol of spermidine formed, linearity not maintained in the absence of DTT | Rattus norvegicus | 5'-methylthioadenosine + spermidine | - |
? | |
| S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane | linear at least up to 6 nmol of spermidine formed, linearity not maintained in the absence of DTT | Rattus norvegicus Wistar | 5'-methylthioadenosine + spermidine | - |
? | |
| S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane | - |
Escherichia coli | 5'-methylthioadenosine + spermidine | - |
? | |
| S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane | - |
Rattus norvegicus | 5'-methylthioadenosine + spermidine | - |
? | |
| S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane | - |
Rattus norvegicus Wistar | 5'-methylthioadenosine + spermidine | - |
? | |
| S-adenosyl-3-methylthio-1-propylamine + 1,5-diaminopentane | - |
Escherichia coli | 5'-methylthioadenosine + N-(3-aminopropyl)-1,5-diaminopentane | aminopropylcadaverine | ? | |
| S-adenosyl-3-methylthio-1-propylamine + 1,5-diaminopentane | 20 times less active than 1,4-diaminobutane at the same concentration | Rattus norvegicus | 5'-methylthioadenosine + N-(3-aminopropyl)-1,5-diaminopentane | - |
? | |
| S-adenosyl-3-methylthio-1-propylamine + 1,5-diaminopentane | 20 times less active than 1,4-diaminobutane at the same concentration | Rattus norvegicus Wistar | 5'-methylthioadenosine + N-(3-aminopropyl)-1,5-diaminopentane | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Escherichia coli |
| dimer | 2 * 37000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI 5.3 | Rattus norvegicus |
| 10 | - |
- |
Rattus norvegicus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
95% loss of activity after 30 min | Rattus norvegicus |
| 10.4 | - |
above, rapid loss of activity | Rattus norvegicus |
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