General Stability | Organism |
---|---|
dialysis against glycine-NaOH buffer, pH 8.8 inactivates | Brassica rapa subsp. pekinensis |
DTT and/or EDTA do not stabilize | Brassica rapa subsp. pekinensis |
very labile in crude extracts | Brassica rapa subsp. pekinensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-deoxy-5'-methylthioadenosine | weak | Brassica rapa subsp. pekinensis | |
5'-deoxy-5'-methylthiotubercidine | weak | Brassica rapa subsp. pekinensis | |
Cyclohexylamine | strong | Brassica rapa subsp. pekinensis | |
S-adenosyl-(5')-3-methylthio-1-propylamine | at concentrations above 0.05 mM | Brassica rapa subsp. pekinensis | |
S-Adenosyl-1,8-diamino-3-thiooctane | transition-state analogue, potent inhibition | Brassica rapa subsp. pekinensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0067 | - |
S-adenosyl-3-methylthio-1-propylamine | pH 8.8, 37ºC, crude extracts | Brassica rapa subsp. pekinensis | |
0.0067 | - |
S-adenosyl-3-methylthio-1-propylamine | pH 7.4, 37ºC, partially purified enzyme | Brassica rapa subsp. pekinensis | |
0.032 | - |
1,4-diaminobutane | pH 8.8, 37ºC, crude extracts | Brassica rapa subsp. pekinensis | |
0.032 | - |
1,4-diaminobutane | pH 7.4, 37ºC, partially purified enzyme | Brassica rapa subsp. pekinensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
81000 | - |
gel filtration | Brassica rapa subsp. pekinensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica rapa subsp. pekinensis | - |
Chinese cabbage, var Pak Choy | - |
Spinacia oleracea | - |
spinach | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation, acetone precipitation and chromatography on Sephadex G-100, 160fold purified | Brassica rapa subsp. pekinensis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Spinacia oleracea | - |
leaf | - |
Brassica rapa subsp. pekinensis | - |
Storage Stability | Organism |
---|---|
-20ºC, 30% loss of activity in 1 month, after final filtration through Sephadex | Brassica rapa subsp. pekinensis |
-20ºC, stable for at least 3 months after acetone precipitation | Brassica rapa subsp. pekinensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane | the reaction is linear from 1 to 10 nmol of spermidine | Brassica rapa subsp. pekinensis | 5'-methylthioadenosine + spermidine | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Spinacia oleracea |
37 | - |
assay at | Brassica rapa subsp. pekinensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
in Gly-NaOH buffer | Brassica rapa subsp. pekinensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
rapidly inactivated at pH more than 9.0 in Gly-NaOH buffer. The activity of the enzyme is generally lower in Tris buffer than in Gly-NaOH buffer | Brassica rapa subsp. pekinensis |