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Literature summary for 2.5.1.16 extracted from
- Propylaminetransferases in chinese cabbage leaves (1984), Plant Physiol., 74, 645-649.
General Stability
| General Stability | Organism |
|---|---|
| dialysis against glycine-NaOH buffer, pH 8.8 inactivates | Brassica rapa subsp. pekinensis |
| DTT and/or EDTA do not stabilize | Brassica rapa subsp. pekinensis |
| very labile in crude extracts | Brassica rapa subsp. pekinensis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5'-deoxy-5'-methylthioadenosine | weak | Brassica rapa subsp. pekinensis |  |
| 5'-deoxy-5'-methylthiotubercidine | weak | Brassica rapa subsp. pekinensis | |
| Cyclohexylamine | strong | Brassica rapa subsp. pekinensis | |
| S-adenosyl-(5')-3-methylthio-1-propylamine | at concentrations above 0.05 mM | Brassica rapa subsp. pekinensis | |
| S-Adenosyl-1,8-diamino-3-thiooctane | transition-state analogue, potent inhibition | Brassica rapa subsp. pekinensis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0067 | - |
S-adenosyl-3-methylthio-1-propylamine | pH 8.8, 37ºC, crude extracts | Brassica rapa subsp. pekinensis | |
| 0.0067 | - |
S-adenosyl-3-methylthio-1-propylamine | pH 7.4, 37ºC, partially purified enzyme | Brassica rapa subsp. pekinensis | |
| 0.032 | - |
1,4-diaminobutane | pH 8.8, 37ºC, crude extracts | Brassica rapa subsp. pekinensis | |
| 0.032 | - |
1,4-diaminobutane | pH 7.4, 37ºC, partially purified enzyme | Brassica rapa subsp. pekinensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 81000 | - |
gel filtration | Brassica rapa subsp. pekinensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brassica rapa subsp. pekinensis | - |
Chinese cabbage, var Pak Choy | - |
| Spinacia oleracea | - |
spinach | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate fractionation, acetone precipitation and chromatography on Sephadex G-100, 160fold purified | Brassica rapa subsp. pekinensis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
| leaf | - |
Brassica rapa subsp. pekinensis | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20ºC, 30% loss of activity in 1 month, after final filtration through Sephadex | Brassica rapa subsp. pekinensis |
| -20ºC, stable for at least 3 months after acetone precipitation | Brassica rapa subsp. pekinensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane | the reaction is linear from 1 to 10 nmol of spermidine | Brassica rapa subsp. pekinensis | 5'-methylthioadenosine + spermidine | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Spinacia oleracea |
| 37 | - |
assay at | Brassica rapa subsp. pekinensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.8 | - |
in Gly-NaOH buffer | Brassica rapa subsp. pekinensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
rapidly inactivated at pH more than 9.0 in Gly-NaOH buffer. The activity of the enzyme is generally lower in Tris buffer than in Gly-NaOH buffer | Brassica rapa subsp. pekinensis |
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Release 2023.1 (January 2023)
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