Cloned (Comment) | Organism |
---|---|
gene cysM, recombinant expression of His-tagged enzyme in Escherichia coli | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-acetyl-L-serine + selenide | Salmonella enterica subsp. enterica serovar Typhimurium | low activity | L-selenocysteine + acetate | - |
? | |
O-acetyl-L-serine + selenide | Salmonella enterica subsp. enterica serovar Typhimurium SGSC1412 | low activity | L-selenocysteine + acetate | - |
? | |
O-acetyl-L-serine + selenide | Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720 | low activity | L-selenocysteine + acetate | - |
? | |
O-acetyl-L-serine + sulfide | Salmonella enterica subsp. enterica serovar Typhimurium | - |
L-cysteine + acetate | - |
? | |
O-acetyl-L-serine + sulfide | Salmonella enterica subsp. enterica serovar Typhimurium SGSC1412 | - |
L-cysteine + acetate | - |
? | |
O-acetyl-L-serine + sulfide | Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720 | - |
L-cysteine + acetate | - |
? | |
O-acetyl-L-serine + sulfite | Salmonella enterica subsp. enterica serovar Typhimurium | - |
L-cysteine sulfonate + acetate | - |
? | |
O-acetyl-L-serine + thiosulfate | Salmonella enterica subsp. enterica serovar Typhimurium | - |
S-sulfo-L-cysteine + acetate | - |
? | |
O-acetyl-L-serine + thiosulfate | Salmonella enterica subsp. enterica serovar Typhimurium SGSC1412 | - |
S-sulfo-L-cysteine + acetate | - |
? | |
O-acetyl-L-serine + thiosulfate | Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720 | - |
S-sulfo-L-cysteine + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | P29848 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720 | P29848 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium SGSC1412 | P29848 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Salmonella enterica subsp. enterica serovar Typhimurium |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
O-acetyl-L-serine + thiosulfate = S-sulfo-L-cysteine + acetate | ping pong bi bi kinetic mechanism | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | development of a rapid and efficient method of synthesis of L-cysteine derivatives containing isotopic sulfur atoms and application of this method to a reactive sulfur metabolome of N-acetyl-L-cysteine (NAC) in HeLa cells. CysM catalyzes reaction with multiple substrates by a ping pong bi bi kinetic mechanism | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
- |
|
additional information | development of a rapid and efficient method of synthesis of L-cysteine derivatives containing isotopic sulfur atoms and application of this method to a reactive sulfur metabolome of N-acetyl-L-cysteine (NAC) in HeLa cells. CysM catalyzes reaction with multiple substrates by a ping pong bi bi kinetic mechanism | Salmonella enterica subsp. enterica serovar Typhimurium SGSC1412 | ? | - |
- |
|
additional information | development of a rapid and efficient method of synthesis of L-cysteine derivatives containing isotopic sulfur atoms and application of this method to a reactive sulfur metabolome of N-acetyl-L-cysteine (NAC) in HeLa cells. CysM catalyzes reaction with multiple substrates by a ping pong bi bi kinetic mechanism | Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720 | ? | - |
- |
|
O-acetyl-L-serine + selenide | - |
Salmonella enterica subsp. enterica serovar Typhimurium | L-selenocysteine + acetate | - |
? | |
O-acetyl-L-serine + selenide | low activity | Salmonella enterica subsp. enterica serovar Typhimurium | L-selenocysteine + acetate | - |
? | |
O-acetyl-L-serine + selenide | - |
Salmonella enterica subsp. enterica serovar Typhimurium SGSC1412 | L-selenocysteine + acetate | - |
? | |
O-acetyl-L-serine + selenide | low activity | Salmonella enterica subsp. enterica serovar Typhimurium SGSC1412 | L-selenocysteine + acetate | - |
? | |
O-acetyl-L-serine + selenide | - |
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720 | L-selenocysteine + acetate | - |
? | |
O-acetyl-L-serine + selenide | low activity | Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720 | L-selenocysteine + acetate | - |
? | |
O-acetyl-L-serine + sulfide | - |
Salmonella enterica subsp. enterica serovar Typhimurium | L-cysteine + acetate | - |
? | |
O-acetyl-L-serine + sulfide | - |
Salmonella enterica subsp. enterica serovar Typhimurium SGSC1412 | L-cysteine + acetate | - |
? | |
O-acetyl-L-serine + sulfide | - |
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720 | L-cysteine + acetate | - |
? | |
O-acetyl-L-serine + sulfite | - |
Salmonella enterica subsp. enterica serovar Typhimurium | L-cysteine sulfonate + acetate | - |
? | |
O-acetyl-L-serine + thiosulfate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | S-sulfo-L-cysteine + acetate | - |
? | |
O-acetyl-L-serine + thiosulfate | - |
Salmonella enterica subsp. enterica serovar Typhimurium SGSC1412 | S-sulfo-L-cysteine + acetate | - |
? | |
O-acetyl-L-serine + thiosulfate | - |
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720 | S-sulfo-L-cysteine + acetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CysM | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
cysteine synthase B | UniProt | Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |