BRENDA - Enzyme Database show
show all sequences of 2.5.1.141

An Escherichia coli cyoE gene homologue in thermophilic Bacillus PS3 encodes a thermotolerant heme O synthase

Saiki, K.; Mogi, T.; Ishizuka, M.; Anraku, Y.; FEBS Lett. 351, 385-388 (1994)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli strain ST4676
Bacillus sp. PS3
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(2E,6E)-farnesyl diphosphate + protoheme IX + H2O
Bacillus sp. PS3
-
diphosphate + ferroheme o
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus sp. PS3
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2E,6E)-farnesyl diphosphate + protoheme IX + H2O
-
742503
Bacillus sp. PS3
diphosphate + ferroheme o
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 25000, SDS-PAGE; x * 32300, calculated from amino acid sequence
Bacillus sp. PS3
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
-
Bacillus sp. PS3
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
37
70
the enzyme retains its activity at 70°C at a level comparable to that at 37°C
Bacillus sp. PS3
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli strain ST4676
Bacillus sp. PS3
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(2E,6E)-farnesyl diphosphate + protoheme IX + H2O
Bacillus sp. PS3
-
diphosphate + ferroheme o
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2E,6E)-farnesyl diphosphate + protoheme IX + H2O
-
742503
Bacillus sp. PS3
diphosphate + ferroheme o
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 25000, SDS-PAGE; x * 32300, calculated from amino acid sequence
Bacillus sp. PS3
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
-
Bacillus sp. PS3
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
37
70
the enzyme retains its activity at 70°C at a level comparable to that at 37°C
Bacillus sp. PS3
Other publictions for EC 2.5.1.141
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743825
Stevens
Cytolytic toxin production by ...
Staphylococcus aureus, Staphylococcus aureus JE2
Sci. Rep.
7
13744
2017
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2
2
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727913
Khalimonchuk
Oligomerization of heme o synt ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae ATCC 204508
J. Biol. Chem.
287
26715-26726
2012
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6
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1
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1
1
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727792
Mogi T.
Over-expression and characteri ...
Bacillus subtilis
J. Biochem.
145
669-675
2009
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1
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727866
Wang
Regulation of the heme A biosy ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae ATCC 204508
J. Biol. Chem.
284
839-847
2009
1
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1
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1
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5
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1
1
1
1
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726940
Brown
Heme O synthase and heme A syn ...
Bacillus subtilis, Bacillus subtilis 168, Rhodobacter sphaeroides, Rhodobacter sphaeroides DSM 158
Biochemistry
43
13541-13548
2004
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2
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9
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2
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2
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727609
Valnot
A mutation in the human heme A ...
Homo sapiens
Hum. Mol. Genet.
9
1245-1249
2000
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1
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1
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3
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1
1
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727597
Murakami
Genomic structure and expressi ...
Homo sapiens
Genomics
42
161-164
1997
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2
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16
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741946
Malakhov
The coxD gene for heme O synt ...
Synechocystis sp. PCC 6803
Biochim. Biophys. Acta
1273
84-86
1996
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4
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1
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728704
Glerum
Isolation of a human cDNA for ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
91
8452-8456
1994
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1
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742503
Saiki
An Escherichia coli cyoE gene ...
Bacillus sp. PS3
FEBS Lett.
351
385-388
1994
-
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1
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1
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3
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1
1
1
1
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1
1
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1
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726848
Saiki
Heme O biosynthesis in Escheri ...
Escherichia coli
Biochem. Biophys. Res. Commun.
189
1491-1497
1993
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727810
Saiki
In vitro heme O synthesis by t ...
Escherichia coli
J. Biol. Chem.
268
26041-26044
1993
1
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2
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1
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1
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727811
Saiki
Identification of the function ...
Escherichia coli
J. Biol. Chem.
268
26927-16934
1993
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40
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3
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1
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40
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1
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743298
Svensson
Bacillus subtilis CtaA and Ct ...
Bacillus subtilis
Mol. Microbiol.
10
193-201
1993
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1
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1
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5
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