Cloned (Comment) | Organism |
---|---|
- |
Staphylococcus aureus |
Crystallization (Comment) | Organism |
---|---|
in presence of O-phospho-L-serine. Formation of the pyridoxal phosphate-alpha-aminoacrylate intermediate induces closure of the active site pocket by narrowing the channel leading to the active site and forming a second substrate binding pocket that likely binds L-glutamate. Active site residues Arg132, Tyr152, Ser185 are essential for O-phospho-L-serine recognition and turnover. Mutations Y152F/S185G induce a closed form of the enzyme in the absence of the alpha-aminoacrylate intermediate | Staphylococcus aureus |
Protein Variants | Comment | Organism |
---|---|---|
R132A | no detectable activity | Staphylococcus aureus |
S185G | about 25% of wild-type activity | Staphylococcus aureus |
Y152F/S185G | completely inactive | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-cysteine | competitive, forms a nonproductive external aldimine with the pyridoxal 5'-phosphate cofactor | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.072 | - |
O-phospho-L-serine | wild-type, pH 8.0, 22°C | Staphylococcus aureus | |
0.22 | - |
O-phospho-L-serine | mutant S185G, pH 8.0, 22°C | Staphylococcus aureus | |
3.2 | - |
L-glutamate | wild-type, pH 8.0, 22°C | Staphylococcus aureus | |
7.1 | - |
L-glutamate | mutant S185G, pH 8.0, 22°C | Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | A6QDA0 | - |
- |
Staphylococcus aureus Newman | A6QDA0 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-phospho-L-serine + L-glutamate | - |
Staphylococcus aureus | N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate + phosphate | - |
? | |
O-phospho-L-serine + L-glutamate | - |
Staphylococcus aureus Newman | N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SbnA | - |
Staphylococcus aureus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
O-phospho-L-serine | mutant S185G, pH 8.0, 22°C | Staphylococcus aureus | |
2 | - |
L-glutamate | mutant S185G, pH 8.0, 22°C | Staphylococcus aureus | |
2.3 | - |
O-phospho-L-serine | wild-type, pH 8.0, 22°C | Staphylococcus aureus | |
3.6 | - |
L-glutamate | wild-type, pH 8.0, 22°C | Staphylococcus aureus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Staphylococcus aureus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Staphylococcus aureus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.29 | - |
L-glutamate | mutant S185G, pH 8.0, 22°C | Staphylococcus aureus | |
1.1 | - |
L-glutamate | wild-type, pH 8.0, 22°C | Staphylococcus aureus | |
7.3 | - |
O-phospho-L-serine | mutant S185G, pH 8.0, 22°C | Staphylococcus aureus | |
32 | - |
O-phospho-L-serine | wild-type, pH 8.0, 22°C | Staphylococcus aureus |