Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 10 mM, increases reaction velocity 1.5fold | Streptomyces anulatus |
Cloned (Comment) | Organism |
---|---|
heterologous expression in Streptomyces coelicolor M512 | Streptomyces anulatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
FeSO4 | 10 mM, completely abolishes the formation of endophenazine A | Streptomyces anulatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
5,10-dihydrophenazine 1-carboxylate | pH 7.5, 30°C | Streptomyces anulatus | |
0.116 | - |
dimethylallyl diphosphate | pH 7.5, 30°C | Streptomyces anulatus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 100 mM MgCl2 increases product formation 1.5fold | Streptomyces anulatus | |
Mg2+ | the enzyme is independent of the presence of magnesium or other divalent cations. 50 mM MgCl2 increases product formation 1.5fold | Streptomyces anulatus | |
Na+ | 500 mM, NaCl increases product formation 3fold | Streptomyces anulatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
x * 37000, SDS-PAGE | Streptomyces anulatus |
37138 | - |
x * 37138, calculated from sequence | Streptomyces anulatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + 5,10-dihydrophenazine 1-carboxylate | Streptomyces anulatus | the enzyme is involved in the biosynthesis of prenylated phenazines | diphosphate + 5,10-dihydro-9-dimethylallylphenazine 1-carboxylate | - |
? | |
dimethylallyl diphosphate + 5,10-dihydrophenazine 1-carboxylate | Streptomyces anulatus 9663 | the enzyme is involved in the biosynthesis of prenylated phenazines | diphosphate + 5,10-dihydro-9-dimethylallylphenazine 1-carboxylate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces anulatus | C4PWA1 | - |
- |
Streptomyces anulatus 9663 | C4PWA1 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces anulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + 5,10-dihydrophenazine 1-carboxylate | the enzyme is involved in the biosynthesis of prenylated phenazines | Streptomyces anulatus | diphosphate + 5,10-dihydro-9-dimethylallylphenazine 1-carboxylate | - |
? | |
dimethylallyl diphosphate + 5,10-dihydrophenazine 1-carboxylate | the enzyme is specific for both dimethylallyl diphosphate and 5,10-dihydrophenazine 1-carboxylate. No activity with geranyldiphosphate or other phenazine substrates, such as phenazine, phenazine 1-carboxylic acid methyl ester, N-methyl-phenazine, or pyocyanine (1-hydroxy-5-N-methyl-phenazine). No activity with 4-hydroxyphenylpyruvate 1,3-dihydroxynaphthalene, and 1,6-dihydroxynaphthalene. Reaction velocity with flaviolin is 0.5% compared to the activity with 5,10-dihydrophenazine 1-carboxylate | Streptomyces anulatus | diphosphate + 5,10-dihydro-9-dimethylallylphenazine 1-carboxylate | - |
? | |
dimethylallyl diphosphate + 5,10-dihydrophenazine 1-carboxylate | the enzyme is involved in the biosynthesis of prenylated phenazines | Streptomyces anulatus 9663 | diphosphate + 5,10-dihydro-9-dimethylallylphenazine 1-carboxylate | - |
? | |
dimethylallyl diphosphate + 5,10-dihydrophenazine 1-carboxylate | the enzyme is specific for both dimethylallyl diphosphate and 5,10-dihydrophenazine 1-carboxylate. No activity with geranyldiphosphate or other phenazine substrates, such as phenazine, phenazine 1-carboxylic acid methyl ester, N-methyl-phenazine, or pyocyanine (1-hydroxy-5-N-methyl-phenazine). No activity with 4-hydroxyphenylpyruvate 1,3-dihydroxynaphthalene, and 1,6-dihydroxynaphthalene. Reaction velocity with flaviolin is 0.5% compared to the activity with 5,10-dihydrophenazine 1-carboxylate | Streptomyces anulatus 9663 | diphosphate + 5,10-dihydro-9-dimethylallylphenazine 1-carboxylate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37000, SDS-PAGE | Streptomyces anulatus |
? | x * 37138, calculated from sequence | Streptomyces anulatus |
Synonyms | Comment | Organism |
---|---|---|
5,10-dihydrophenazine 1-carboxylate dimethylallyltransferase | - |
Streptomyces anulatus |
dihydrophenazine-1-carboxylate dimethylallyltransferase | - |
Streptomyces anulatus |
Ppzp | - |
Streptomyces anulatus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.435 | - |
5,10-dihydrophenazine 1-carboxylate | pH 7.5, 30°C | Streptomyces anulatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Streptomyces anulatus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9.5 | pH 5.0: about 50% of maximal activity, ph 9.5: about 50% of maximal activity | Streptomyces anulatus |
General Information | Comment | Organism |
---|---|---|
malfunction | after inactivation of ppzP, only nonprenylated phenazine 1-carboxylic acid is formed | Streptomyces anulatus |
physiological function | the enzyme is involved in the biosynthesis of prenylated phenazines | Streptomyces anulatus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.4 | - |
5,10-dihydrophenazine 1-carboxylate | pH 7.5, 30°C | Streptomyces anulatus |