Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.120 extracted from

  • Mahanta, N.; Fedoseyenko, D.; Dairi, T.; Begley, T.P.
    Menaquinone biosynthesis: formation of aminofutalosine requires a unique radical SAM enzyme (2013), J. Am. Chem. Soc., 135, 15318-15321.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
cloned into the pQE-30 expression vector, and overexpressed in Escherichia coli M15 (pREP4) Thermus thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
[4Fe-4S] cluster presence of the predicted [4Fe-4S] cluster. Iron and sulfide analysis yields 2.4 irons and 2.8 sulfides per monomer of MqnE, indicating incomplete reconstitution of the cluster Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 3-[(1-carboxyvinyl)oxy]benzoate + H2O Thermus thermophilus the enzyme is part of the futalosine pathway for menaquinone biosynthesis 6-amino-6-deoxyfutalosine + L-methionine + bicarbonate 6-amino-6-deoxyfutalosine i.e. 3-[3-[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]propanoyl]benzoate ?

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q5SK48
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 3-[(1-carboxyvinyl)oxy]benzoate + H2O
-
Thermus thermophilus 6-amino-6-deoxyfutalosine + L-methionine + bicarbonate 6-amino-6-deoxyfutalosine i.e. 3-[3-[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]propanoyl]benzoate ?
S-adenosyl-L-methionine + 3-[(1-carboxyvinyl)oxy]benzoate + H2O the enzyme is part of the futalosine pathway for menaquinone biosynthesis Thermus thermophilus 6-amino-6-deoxyfutalosine + L-methionine + bicarbonate 6-amino-6-deoxyfutalosine i.e. 3-[3-[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]propanoyl]benzoate ?

Synonyms

Synonyms Comment Organism
AFL synthase
-
Thermus thermophilus
aminofutalosine synthase
-
Thermus thermophilus
MqnE
-
Thermus thermophilus
TTHA0804 locus name Thermus thermophilus

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine the enzyme is a member of the AdoMet radical family. S-Adenosyl-L-methionine acts as both a radical generator and as the source of the transferred adenosyl group Thermus thermophilus

General Information

General Information Comment Organism
physiological function the enzyme is part of the futalosine pathway for menaquinone biosynthesis Thermus thermophilus