Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli M15[pREP4] cells | Humulus lupulus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0162 | - |
ATP | in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C | Humulus lupulus | |
0.0193 | - |
ADP | in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C | Humulus lupulus | |
0.0195 | - |
dimethylallyl diphosphate | in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C | Humulus lupulus | |
0.759 | - |
AMP | in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C | Humulus lupulus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36693 | - |
x * 36693, calculated from amino acid sequence | Humulus lupulus |
37000 | - |
x * 37000, SDS-PAGE | Humulus lupulus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + ADP | Humulus lupulus | - |
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate | - |
? | |
dimethylallyl diphosphate + ATP | Humulus lupulus | ATP is the best cosubstrate | diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Humulus lupulus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-chelate affinity column chromatography | Humulus lupulus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cone | - |
Humulus lupulus | - |
leaf | - |
Humulus lupulus | - |
root | - |
Humulus lupulus | - |
stem | - |
Humulus lupulus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + ADP | - |
Humulus lupulus | diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate | - |
? | |
dimethylallyl diphosphate + AMP | low activity with AMP | Humulus lupulus | diphosphate + N6-(dimethylallyl)adenosine phosphate | - |
? | |
dimethylallyl diphosphate + ATP | ATP is the best cosubstrate | Humulus lupulus | diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate | - |
? | |
additional information | GMP, IMP, CMP, and UMP are not accepted as a substrate | Humulus lupulus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37000, SDS-PAGE | Humulus lupulus |
? | x * 36693, calculated from amino acid sequence | Humulus lupulus |
Synonyms | Comment | Organism |
---|---|---|
adenylate isopentenyltransferase | - |
Humulus lupulus |
AIPT | - |
Humulus lupulus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
AMP | in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C | Humulus lupulus | |
0.022 | - |
ATP | in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C | Humulus lupulus | |
0.024 | - |
ADP | in 100 mM TrisHCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C | Humulus lupulus | |
0.027 | - |
dimethylallyl diphosphate | in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C | Humulus lupulus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | preferred ligand over AMP | Humulus lupulus | |
ATP | preferred ligand over AMP | Humulus lupulus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0109 | - |
AMP | in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C | Humulus lupulus | |
1.238 | - |
ADP | in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C | Humulus lupulus | |
1.34 | - |
ATP | in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C | Humulus lupulus | |
1.397 | - |
dimethylallyl diphosphate | in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C | Humulus lupulus |