BRENDA - Enzyme Database
show all sequences of 2.5.1.108

Diphthamide modification of eEF2 requires a J-domain protein and is essential for normal development

Webb, T.R.; Cross, S.H.; McKie, L.; Edgar, R.; Vizor, L.; Harrison, J.; Peters, J.; Jackson, I.J.; J. Cell Sci. 121, 3140-3145 (2008)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli and in NIH-3T3 cells
Mus musculus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoskeleton
-
Mus musculus
5856
-
Organism
Organism
UniProt
Commentary
Textmining
Mus musculus
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
embryo
-
Mus musculus
-
Synonyms
Synonyms
Commentary
Organism
DPH4
-
Mus musculus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli and in NIH-3T3 cells
Mus musculus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoskeleton
-
Mus musculus
5856
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
embryo
-
Mus musculus
-
General Information
General Information
Commentary
Organism
malfunction
loss of Dph4 results in lack of diphthamide modification of eEF2 and resistance to diphtheria toxin. Dph4 mutant mice are growth retarded and have a specific digit defect with one or more additional preaxial digits
Mus musculus
General Information (protein specific)
General Information
Commentary
Organism
malfunction
loss of Dph4 results in lack of diphthamide modification of eEF2 and resistance to diphtheria toxin. Dph4 mutant mice are growth retarded and have a specific digit defect with one or more additional preaxial digits
Mus musculus
Other publictions for EC 2.5.1.108
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738461
Dong
An organometallic complex form ...
Pyrococcus horikoshii
J. Am. Chem. Soc.
138
9755-9758
2016
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-
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-
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2
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2
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1
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1
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1
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-
2
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
739730
Abdel-Fattah
Insights into diphthamide, key ...
Saccharomyces cerevisiae
Toxins
5
958-968
2013
-
-
1
-
-
-
-
-
-
-
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-
1
-
-
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-
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1
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1
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-
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1
1
-
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723158
Zhu
Mechanistic understanding of P ...
Pyrococcus horikoshii
Mol. Biosyst.
7
74-81
2011
-
-
1
1
8
-
-
-
-
2
1
2
-
4
-
-
2
-
-
-
-
-
4
2
2
1
-
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1
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1
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1
8
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2
1
2
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2
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4
2
1
-
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-
1
-
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1
1
-
-
-
723263
Zhang
Lin, H.: Diphthamide biosynthe ...
Pyrococcus horikoshii
Nature
465
891-896
2010
-
-
2
2
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2
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2
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1
-
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1
1
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3
1
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2
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2
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2
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2
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1
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3
1
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-
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1
1
-
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-
725594
Webb
Diphthamide modification of eE ...
Mus musculus
J. Cell Sci.
121
3140-3145
2008
-
-
1
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
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1
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1
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1
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1
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-
-
-
-
-
1
1
-
-
-
718109
Liu
Identification of the proteins ...
Saccharomyces cerevisiae, Mus musculus
Mol. Cell. Biol.
24
9487-9497
2004
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2
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2
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1
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2
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2
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2
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2
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4
4
-
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-
725093
Mattheakis
Diphthamide synthesis in Sacch ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae FY251
Gene
132
149-154
1993
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-
1
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2
2
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2
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2
2
2
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1
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1
1
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2
2
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2
2
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