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Literature summary for 2.5.1.106 extracted from

  • Zhou, K.; Zhao, W.; Liu, X.; Li, S.
    Saturation mutagenesis on Tyr205 of the cyclic dipeptide C2-prenyltransferase FtmPT1 results in mutants with strongly increased C3-prenylating activity (2016), Appl. Microbiol. Biotechnol., 100, 943-9953.
    View publication on PubMed
Search for more literature for 2.5.1.106

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Aspergillus fumigatus

Protein Variants

Protein Variants Comment Organism
Y205A mutant produces 70.3% C3-regularly prenylated product Aspergillus fumigatus
Y205C mutant produces 39.5% C3-regularly prenylated product Aspergillus fumigatus
Y205D mutant produces 55.8% C3-regularly prenylated product and 3.6% C3-reversely prenylated product Aspergillus fumigatus
Y205E mutant produces 57.6% C3-regularly prenylated product and 3% C3-reversely prenylated product Aspergillus fumigatus
Y205F mutant produces 0.04% C3-regularly prenylated product Aspergillus fumigatus
Y205G mutant produces 92% C3-regularly prenylated product and 12.2% C3-reversely prenylated product Aspergillus fumigatus
Y205H mutant produces 60.7% C3-regularly prenylated product Aspergillus fumigatus
Y205I mutant produces 39.5% C3-regularly prenylated product Aspergillus fumigatus
Y205K mutant produces 110% C3-regularly prenylated product and 1.6% C3-reversely prenylated product Aspergillus fumigatus
Y205L mutant produces 56.4% C3-regularly prenylated product Aspergillus fumigatus
Y205M mutant produces 9.8% C3-regularly prenylated product Aspergillus fumigatus
Y205N mutant produces 70% C3-regularly prenylated product and 2.8% C3-reversely prenylated product Aspergillus fumigatus
Y205P mutant produces 38.7% C3-regularly prenylated product Aspergillus fumigatus
Y205Q mutant produces 33.7% C3-regularly prenylated product Aspergillus fumigatus
Y205R mutant produces 77% C3-regularly prenylated product and 13.2% C3-reversely prenylated product Aspergillus fumigatus
Y205S mutant produces 50.4% C3-regularly prenylated product and 1.8% C3-reversely prenylated product Aspergillus fumigatus
Y205T mutant does not produce C3-regularly prenylated product Aspergillus fumigatus
Y205V mutant produces 33.0% C3-regularly prenylated product Aspergillus fumigatus
Y205W mutant does not produce C3-regularly prenylated product Aspergillus fumigatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.057
-
 cyclo-(L-Trp-L-Pro) wild-type, pH 7.5, 37°C Aspergillus fumigatus
0.083
-
 cyclo-(L-Trp-D-Pro) mutant Y205L, pH 7.5, 37°C Aspergillus fumigatus
0.11
-
 cyclo-(L-Trp-L-Pro) mutant Y205N, pH 7.5, 37°C Aspergillus fumigatus
0.19
-
 cyclo-(L-Trp-L-Pro) mutant Y205L, pH 7.5, 37°C Aspergillus fumigatus
0.28
-
 cyclo-(D-Trp-D-Pro) mutant Y205L, pH 7.5, 37°C Aspergillus fumigatus
0.32
-
 cyclo-(D-Trp-D-Pro) mutant Y205N, pH 7.5, 37°C Aspergillus fumigatus
0.4
-
 cyclo-(D-Trp-L-Pro) mutant Y205N, pH 7.5, 37°C Aspergillus fumigatus
0.46
-
 cyclo-(D-Trp-L-Pro) mutant Y205L, pH 7.5, 37°C Aspergillus fumigatus
0.62
-
 cyclo-(L-Trp-D-Pro) mutant Y205N, pH 7.5, 37°C Aspergillus fumigatus

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus Q4WAW7
-
-
Aspergillus fumigatus ATCC MYA-4609 Q4WAW7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate + brevianamide F
-
 Aspergillus fumigatus diphosphate + tryprostatin B
-
 ?
dimethylallyl diphosphate + brevianamide F
-
 Aspergillus fumigatus ATCC MYA-4609 diphosphate + tryprostatin B
-
 ?
dimethylallyl diphosphate + brevianamide F
-
 Aspergillus fumigatus diphosphate + (5aS,6aR,11aR,13aS)-6a-(3-methylbut-2-en-1-yl)-2,3,6,6a,11,11a-hexahydro-1H-pyrrolo[1'',2'':4',5']pyrazino[1',2':1,5]pyrrolo[2,3-b]indole-5,13(5aH,13aH)-dione product of Tyr205 mutants, no product of wild-type ?
dimethylallyl diphosphate + brevianamide F
-
 Aspergillus fumigatus ATCC MYA-4609 diphosphate + (5aS,6aR,11aR,13aS)-6a-(3-methylbut-2-en-1-yl)-2,3,6,6a,11,11a-hexahydro-1H-pyrrolo[1'',2'':4',5']pyrazino[1',2':1,5]pyrrolo[2,3-b]indole-5,13(5aH,13aH)-dione product of Tyr205 mutants, no product of wild-type ?
dimethylallyl diphosphate + cyclo-(D-Trp-D-Pro)
-
 Aspergillus fumigatus diphosphate + cyclo-(2-dimethylallyl-D-Trp-D-Pro)
-
 ?
dimethylallyl diphosphate + cyclo-(D-Trp-D-Pro)
-
 Aspergillus fumigatus ATCC MYA-4609 diphosphate + cyclo-(2-dimethylallyl-D-Trp-D-Pro)
-
 ?
dimethylallyl diphosphate + cyclo-(D-Trp-L-Pro)
-
 Aspergillus fumigatus diphosphate + cyclo-(2-dimethylallyl-Trp-L-Pro)
-
 ?
dimethylallyl diphosphate + cyclo-(D-Trp-L-Pro)
-
 Aspergillus fumigatus ATCC MYA-4609 diphosphate + cyclo-(2-dimethylallyl-Trp-L-Pro)
-
 ?
dimethylallyl diphosphate + cyclo-(L-Trp-D-Pro)
-
 Aspergillus fumigatus diphosphate + cyclo-(2-dimethylallyl-L-Trp-D-Pro)
-
 ?
dimethylallyl diphosphate + cyclo-(L-Trp-D-Pro)
-
 Aspergillus fumigatus ATCC MYA-4609 diphosphate + cyclo-(2-dimethylallyl-L-Trp-D-Pro)
-
 ?
dimethylallyl diphosphate + cyclo-(L-Trp-L-Pro)
-
 Aspergillus fumigatus diphosphate + cyclo-(2-dimethylallyl-Trp-L-Pro)
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.18
-
 cyclo-(L-Trp-D-Pro) mutant Y205L, pH 7.5, 37°C Aspergillus fumigatus
0.21
-
 cyclo-(D-Trp-L-Pro) mutant Y205N, pH 7.5, 37°C Aspergillus fumigatus
0.25
-
 cyclo-(D-Trp-D-Pro) mutant Y205N, pH 7.5, 37°C Aspergillus fumigatus
0.32
-
 cyclo-(L-Trp-D-Pro) mutant Y205N, pH 7.5, 37°C Aspergillus fumigatus
0.41
-
 cyclo-(D-Trp-L-Pro) mutant Y205L, pH 7.5, 37°C Aspergillus fumigatus
1.36
-
 cyclo-(L-Trp-L-Pro) mutant Y205N, pH 7.5, 37°C Aspergillus fumigatus
1.39
-
 cyclo-(D-Trp-D-Pro) mutant Y205L, pH 7.5, 37°C Aspergillus fumigatus
4.26
-
 cyclo-(L-Trp-L-Pro) mutant Y205L, pH 7.5, 37°C Aspergillus fumigatus
8.9
-
 cyclo-(L-Trp-L-Pro) wild-type, pH 7.5, 37°C Aspergillus fumigatus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.52
-
 cyclo-(L-Trp-D-Pro) mutant Y205N, pH 7.5, 37°C Aspergillus fumigatus
0.53
-
 cyclo-(D-Trp-L-Pro) mutant Y205N, pH 7.5, 37°C Aspergillus fumigatus
0.78
-
 cyclo-(D-Trp-D-Pro) mutant Y205N, pH 7.5, 37°C Aspergillus fumigatus
0.89
-
 cyclo-(D-Trp-L-Pro) mutant Y205L, pH 7.5, 37°C Aspergillus fumigatus
2.2
-
 cyclo-(L-Trp-D-Pro) mutant Y205L, pH 7.5, 37°C Aspergillus fumigatus
4.9
-
 cyclo-(D-Trp-D-Pro) mutant Y205L, pH 7.5, 37°C Aspergillus fumigatus
12
-
 cyclo-(L-Trp-L-Pro) mutant Y205N, pH 7.5, 37°C Aspergillus fumigatus
22
-
 cyclo-(L-Trp-L-Pro) mutant Y205L, pH 7.5, 37°C Aspergillus fumigatus
156
-
 cyclo-(L-Trp-L-Pro) wild-type, pH 7.5, 37°C Aspergillus fumigatus