Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | not activated by Tween 20, Triton X-100, Chaps or Nonidet P-40 | Abies grandis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphate | 0.66 mM, 50% inhibition | Abies grandis | |
farnesyl diphosphate | 0.06 mM, 50% inhibition | Abies grandis | |
K+ | 500 mM, 63% inhibition in the presence of MgCl2 | Abies grandis | |
Mn2+ | strong inhibition above 0.1 mM, activation below | Abies grandis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0018 | - |
geranyl diphosphate | - |
Abies grandis | |
0.009 | - |
dimethylallyl diphosphate | - |
Abies grandis | |
0.0153 | - |
isopentenyl diphosphate | - |
Abies grandis | |
0.0445 | - |
Mn2+ | - |
Abies grandis | |
0.0712 | - |
Mg2+ | - |
Abies grandis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 18% of Mg2+ activation at 0.5 mM, inhibition above | Abies grandis | |
Co2+ | 78% of Mg2+ activation at 1 mM | Abies grandis | |
Mg2+ | maximal activity at 1 mM | Abies grandis | |
Mg2+ | required for activity | Abies grandis | |
Mn2+ | can replace Mg2+, maximal activity at 0.1 mM, inhibition above | Abies grandis | |
Ni2+ | 10% of Mg2+ activation at 0.5 mM, inhibition above | Abies grandis | |
Zn2+ | 91% of Mg2+ activation at 0.5 mM, almost complete inhibition at 5 mM | Abies grandis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
gel filtration | Abies grandis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Abies grandis | - |
grand fir | - |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose, Mono Q, phenyl-Sepharose | Abies grandis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
sapling | - |
Abies grandis | - |
Storage Stability | Organism |
---|---|
-80°C, 3 months, no loss of activity | Abies grandis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + 2 isopentenyl diphosphate | - |
Abies grandis | (E,E)-farnesyl diphosphate + 2 diphosphate | - |
? | |
geranyl diphosphate + isopentenyl diphosphate | - |
Abies grandis | diphosphate + (2E,6E)-farnesyl diphosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Abies grandis |