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Literature summary for 2.5.1.10 extracted from

  • Tholl, D.; Croteau, R.; Gershenzon, J.
    Partial purification and characterization of the short-chain prenyltransferases, gernayl diphospate synthase and farnesyl diphosphate synthase, from Abies grandis (grand fir) (2001), Arch. Biochem. Biophys., 386, 233-242.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information not activated by Tween 20, Triton X-100, Chaps or Nonidet P-40 Abies grandis

Inhibitors

Inhibitors Comment Organism Structure
diphosphate 0.66 mM, 50% inhibition Abies grandis
farnesyl diphosphate 0.06 mM, 50% inhibition Abies grandis
K+ 500 mM, 63% inhibition in the presence of MgCl2 Abies grandis
Mn2+ strong inhibition above 0.1 mM, activation below Abies grandis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0018
-
geranyl diphosphate
-
Abies grandis
0.009
-
dimethylallyl diphosphate
-
Abies grandis
0.0153
-
isopentenyl diphosphate
-
Abies grandis
0.0445
-
Mn2+
-
Abies grandis
0.0712
-
Mg2+
-
Abies grandis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 18% of Mg2+ activation at 0.5 mM, inhibition above Abies grandis
Co2+ 78% of Mg2+ activation at 1 mM Abies grandis
Mg2+ maximal activity at 1 mM Abies grandis
Mg2+ required for activity Abies grandis
Mn2+ can replace Mg2+, maximal activity at 0.1 mM, inhibition above Abies grandis
Ni2+ 10% of Mg2+ activation at 0.5 mM, inhibition above Abies grandis
Zn2+ 91% of Mg2+ activation at 0.5 mM, almost complete inhibition at 5 mM Abies grandis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
gel filtration Abies grandis

Organism

Organism UniProt Comment Textmining
Abies grandis
-
grand fir
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose, Mono Q, phenyl-Sepharose Abies grandis

Source Tissue

Source Tissue Comment Organism Textmining
sapling
-
Abies grandis
-

Storage Stability

Storage Stability Organism
-80°C, 3 months, no loss of activity Abies grandis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate + 2 isopentenyl diphosphate
-
Abies grandis (E,E)-farnesyl diphosphate + 2 diphosphate
-
?
geranyl diphosphate + isopentenyl diphosphate
-
Abies grandis diphosphate + (2E,6E)-farnesyl diphosphate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
Abies grandis