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Literature summary for 2.5.1.10 extracted from
- Prenyltransferase from human liver (1985), Methods Enzymol., 110, 155-171.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithioerythritol | - |
Homo sapiens |  |
General Stability
| General Stability | Organism |
|---|---|
| thiol reducing agents are detrimental for stability | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| isopentenyl diphosphate | - |
Homo sapiens | |
| Phenylglyoxal | - |
Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for activity | Homo sapiens | |
| Mn2+ | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 74000 | - |
gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| geranyl diphosphate + isopentenyl diphosphate = diphosphate + (2E,6E)-farnesyl diphosphate | bimolecular nucleophilic substitution, SN2 reaction, proceeds with an inversion of configuration at the diphosphate-bearing carbon of the allylic substrate and removal of the pro-R hydrogen from the C-2 of isopentenyl diphosphate in these trans-polyprenol synthetic reactions | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.922 | - |
- |
Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, precipitate in neutral (NH4)2SO4 solution at 60% saturation, 1 year, 50-70% loss of activity | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylallyl diphosphate + 2 isopentenyl diphosphate | 2 sequential irreversible 1', 4 condensations | Homo sapiens | (E,E)-farnesyl diphosphate + 2 diphosphate | - |
ir | |
| geranyl diphosphate + isopentenyl diphosphate | - |
Homo sapiens | diphosphate + farnesyl diphosphate | - |
ir | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | 8.8 | - |
Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0007 | - |
isopentenyl diphosphate | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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