Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | 8 mM, 33% inhibition | Ricinus communis | |
Mn2+ | activates at 0.25-0.50 mM, inhibition above | Ricinus communis | |
additional information | neither iodoacetamide nor N-ethylmaleimide inhibits at concentration less than 5 mM | Ricinus communis | |
N-ethylmaleimide | 8 mM, 54% inhibition | Ricinus communis | |
p-hydroxymercuribenzoate | 1 mM, 25% inhibition | Ricinus communis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0045 | - |
dimethylallyl diphosphate | 0.042-0.056 mg/ml protein, isoenzyme II, Km values depend on protein concentration | Ricinus communis | |
0.0059 | - |
dimethylallyl diphosphate | 0.042-0.056 mg/ml protein, isoenzyme I, Km values depend on protein concentration | Ricinus communis | |
0.032 | - |
isopentenyl diphosphate | 0.042-0.056 mg/ml protein, isoenzyme II, Km values depend on protein concentration | Ricinus communis | |
0.036 | - |
geranyl diphosphate | 0.042-0.056 mg/ml protein, isoenzyme I, Km values depend on protein concentration | Ricinus communis | |
0.039 | - |
geranyl diphosphate | 0.042-0.056 mg/ml protein, isoenzyme II, Km values depend on protein concentration | Ricinus communis | |
0.048 | - |
isopentenyl diphosphate | 0.042-0.056 mg/ml protein, isoenzyme I, Km values depend on protein concentration | Ricinus communis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Ricinus communis | |
Mg2+ | maximum activity at 1-2 mM | Ricinus communis | |
Mn2+ | activation at 0.25-0.50 mM, inhibition above | Ricinus communis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
gel filtration, enzyme I, protein concentration 0.02 mg/ml | Ricinus communis |
60000 | - |
gel filtration, enzyme II, protein concentration 0.02 mg/ml | Ricinus communis |
72500 | - |
gel filtration, protein concentration 25 mg/ml | Ricinus communis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ricinus communis | - |
isoenzyme I and II | - |
Purification (Comment) | Organism |
---|---|
- |
Ricinus communis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.04 | - |
isoenzyme II | Ricinus communis |
0.06 | - |
isoenzyme I | Ricinus communis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + 2 isopentenyl diphosphate | - |
Ricinus communis | (E,E)-farnesyl diphosphate + 2 diphosphate | - |
? | |
geranyl diphosphate + isopentenyl diphosphate | - |
Ricinus communis | diphosphate + farnesyl diphosphate | E)-farnesyl diphosphate | ? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
- |
Ricinus communis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.5 | approx. 45% of maximal activity at pH 5.5, approx. 60% of activity maximum at pH 8.5, emzyme I | Ricinus communis |