BRENDA - Enzyme Database show
show all sequences of 2.4.99.B5

Identification, cloning, expression, and characterization of the extracellular acarbose-modifying glycosyltransferase, AcbD, from Actinoplanes sp. strain SE50

Hemker, M.; Stratmann, A.; Goeke, K.; Schröder, W.; Lenz, J.; Piepersberg, W.; Pape, H.; J. Bacteriol. 183, 4484-4492 (2001)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
heterologous expression in Streptomyces lividans TK23
Actinoplanes sp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
-
Actinoplanes sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.65
-
acarbose
pH 6.3, 30°C
Actinoplanes sp.
0.96
-
maltose
pH 6.3, 30°C
Actinoplanes sp.
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
-
Actinoplanes sp.
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
required
Actinoplanes sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
76000
-
x * 76000, SDS-PAGE
Actinoplanes sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acarbose + maltotriose
Actinoplanes sp.
the enzyme is involved in the extracellular formation of the series of acarbose-homologous compounds
acarviosyl-alpha-(1-4)-maltotriose + maltose
-
-
?
acarbose + maltotriose
Actinoplanes sp. 50/110
the enzyme is involved in the extracellular formation of the series of acarbose-homologous compounds
acarviosyl-alpha-(1-4)-maltotriose + maltose
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Actinoplanes sp.
Q27GR5
-
-
Actinoplanes sp. 50/110
Q27GR5
-
-
Purification (Commentary)
Commentary
Organism
-
Actinoplanes sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acarbose + cellobiose
-
729911
Actinoplanes sp.
acarviosyl-(1->4)-alpha-cellobiose + maltose
-
-
-
?
acarbose + cellobiose
-
729911
Actinoplanes sp. 50/110
acarviosyl-(1->4)-alpha-cellobiose + maltose
-
-
-
?
acarbose + laminaribiose
-
729911
Actinoplanes sp.
acarviosyl-(1->4)-alpha-laminaribiose + maltose
-
-
-
?
acarbose + laminaribiose
-
729911
Actinoplanes sp. 50/110
acarviosyl-(1->4)-alpha-laminaribiose + maltose
-
-
-
?
acarbose + maltoheptaose
-
729911
Actinoplanes sp.
acarviosyl-(1->4)-alpha-maltoheptaose + maltose
-
-
-
?
acarbose + maltoheptaose
-
729911
Actinoplanes sp. 50/110
acarviosyl-(1->4)-alpha-maltoheptaose + maltose
-
-
-
?
acarbose + maltohexaose
-
729911
Actinoplanes sp.
acarviosyl-(1->4)-alpha-maltohexaose + maltose
-
-
-
?
acarbose + maltopentaose
-
729911
Actinoplanes sp.
acarviosyl-(1->4)-alpha-maltopentaose + maltose
-
-
-
?
acarbose + maltose
-
729911
Actinoplanes sp.
acarviosyl-alpha-(1->4)-maltose + maltose
-
-
-
?
acarbose + maltotetraose
-
729911
Actinoplanes sp.
acarviosyl-(1->4)-alpha-maltotetraose + maltose
-
-
-
?
acarbose + maltotriose
the enzyme is involved in the extracellular formation of the series of acarbose-homologous compounds
729911
Actinoplanes sp.
acarviosyl-alpha-(1-4)-maltotriose + maltose
-
-
-
?
acarbose + maltotriose
the enzyme is involved in the extracellular formation of the series of acarbose-homologous compounds
729911
Actinoplanes sp. 50/110
acarviosyl-alpha-(1-4)-maltotriose + maltose
-
-
-
?
acarbose + maltotriose
-
729911
Actinoplanes sp.
acarviosyl-alpha-(1->4)-maltotriose + maltose
-
-
-
?
acarbose + nonyl alpha-D-glucopyranoside
-
729911
Actinoplanes sp.
nonyl acarviosyl-(1->4)-alpha-D-glucopyranoside + maltose
-
-
-
?
acarbose + xylobiose
-
729911
Actinoplanes sp.
acarviosyl-(1->4)-alpha-xylobiose + maltose
-
-
-
?
acarviosyl glucose + maltose
-
729911
Actinoplanes sp.
acarviosyl-alpha-(1->4)-maltose + D-glucose
-
-
-
?
acarviosyl-alpha-(1->4)-Glc-alpha-(1->4)-Glc-alpha-(1->4)-alpha-Glc + maltose
-
729911
Actinoplanes sp.
acarviosyl-maltose + Glc-alpha-(1->4)-Glc-alpha-(1->4)-Glc
-
-
-
?
additional information
a wide range of additional donor and acceptor substrates are determined for the enzyme. Acceptors reveal a structural requirement for glucose-analogous structures conserving only the overall stereochemistry, except for the anomeric C atom, and the hydroxyl groups at positions 2, 3, and 4 of D-glucose. The enzyme shows neither alpha-amylase nor cyclodextrin glucanotransferase activity
729911
Actinoplanes sp.
?
-
-
-
-
additional information
a wide range of additional donor and acceptor substrates are determined for the enzyme. Acceptors reveal a structural requirement for glucose-analogous structures conserving only the overall stereochemistry, except for the anomeric C atom, and the hydroxyl groups at positions 2, 3, and 4 of D-glucose. The enzyme shows neither alpha-amylase nor cyclodextrin glucanotransferase activity
729911
Actinoplanes sp. 50/110
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 76000, SDS-PAGE
Actinoplanes sp.
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Actinoplanes sp.
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
thermostable up to 40°C
Actinoplanes sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.2
6.9
-
Actinoplanes sp.
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Actinoplanes sp.
isoelectric focusing, pH gradient from 3 to 10
-
5
Cloned(Commentary) (protein specific)
Commentary
Organism
heterologous expression in Streptomyces lividans TK23
Actinoplanes sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
-
Actinoplanes sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.65
-
acarbose
pH 6.3, 30°C
Actinoplanes sp.
0.96
-
maltose
pH 6.3, 30°C
Actinoplanes sp.
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
-
Actinoplanes sp.
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
required
Actinoplanes sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
76000
-
x * 76000, SDS-PAGE
Actinoplanes sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acarbose + maltotriose
Actinoplanes sp.
the enzyme is involved in the extracellular formation of the series of acarbose-homologous compounds
acarviosyl-alpha-(1-4)-maltotriose + maltose
-
-
?
acarbose + maltotriose
Actinoplanes sp. 50/110
the enzyme is involved in the extracellular formation of the series of acarbose-homologous compounds
acarviosyl-alpha-(1-4)-maltotriose + maltose
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Actinoplanes sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acarbose + cellobiose
-
729911
Actinoplanes sp.
acarviosyl-(1->4)-alpha-cellobiose + maltose
-
-
-
?
acarbose + cellobiose
-
729911
Actinoplanes sp. 50/110
acarviosyl-(1->4)-alpha-cellobiose + maltose
-
-
-
?
acarbose + laminaribiose
-
729911
Actinoplanes sp.
acarviosyl-(1->4)-alpha-laminaribiose + maltose
-
-
-
?
acarbose + laminaribiose
-
729911
Actinoplanes sp. 50/110
acarviosyl-(1->4)-alpha-laminaribiose + maltose
-
-
-
?
acarbose + maltoheptaose
-
729911
Actinoplanes sp.
acarviosyl-(1->4)-alpha-maltoheptaose + maltose
-
-
-
?
acarbose + maltoheptaose
-
729911
Actinoplanes sp. 50/110
acarviosyl-(1->4)-alpha-maltoheptaose + maltose
-
-
-
?
acarbose + maltohexaose
-
729911
Actinoplanes sp.
acarviosyl-(1->4)-alpha-maltohexaose + maltose
-
-
-
?
acarbose + maltopentaose
-
729911
Actinoplanes sp.
acarviosyl-(1->4)-alpha-maltopentaose + maltose
-
-
-
?
acarbose + maltose
-
729911
Actinoplanes sp.
acarviosyl-alpha-(1->4)-maltose + maltose
-
-
-
?
acarbose + maltotetraose
-
729911
Actinoplanes sp.
acarviosyl-(1->4)-alpha-maltotetraose + maltose
-
-
-
?
acarbose + maltotriose
the enzyme is involved in the extracellular formation of the series of acarbose-homologous compounds
729911
Actinoplanes sp.
acarviosyl-alpha-(1-4)-maltotriose + maltose
-
-
-
?
acarbose + maltotriose
the enzyme is involved in the extracellular formation of the series of acarbose-homologous compounds
729911
Actinoplanes sp. 50/110
acarviosyl-alpha-(1-4)-maltotriose + maltose
-
-
-
?
acarbose + maltotriose
-
729911
Actinoplanes sp.
acarviosyl-alpha-(1->4)-maltotriose + maltose
-
-
-
?
acarbose + nonyl alpha-D-glucopyranoside
-
729911
Actinoplanes sp.
nonyl acarviosyl-(1->4)-alpha-D-glucopyranoside + maltose
-
-
-
?
acarbose + xylobiose
-
729911
Actinoplanes sp.
acarviosyl-(1->4)-alpha-xylobiose + maltose
-
-
-
?
acarviosyl glucose + maltose
-
729911
Actinoplanes sp.
acarviosyl-alpha-(1->4)-maltose + D-glucose
-
-
-
?
acarviosyl-alpha-(1->4)-Glc-alpha-(1->4)-Glc-alpha-(1->4)-alpha-Glc + maltose
-
729911
Actinoplanes sp.
acarviosyl-maltose + Glc-alpha-(1->4)-Glc-alpha-(1->4)-Glc
-
-
-
?
additional information
a wide range of additional donor and acceptor substrates are determined for the enzyme. Acceptors reveal a structural requirement for glucose-analogous structures conserving only the overall stereochemistry, except for the anomeric C atom, and the hydroxyl groups at positions 2, 3, and 4 of D-glucose. The enzyme shows neither alpha-amylase nor cyclodextrin glucanotransferase activity
729911
Actinoplanes sp.
?
-
-
-
-
additional information
a wide range of additional donor and acceptor substrates are determined for the enzyme. Acceptors reveal a structural requirement for glucose-analogous structures conserving only the overall stereochemistry, except for the anomeric C atom, and the hydroxyl groups at positions 2, 3, and 4 of D-glucose. The enzyme shows neither alpha-amylase nor cyclodextrin glucanotransferase activity
729911
Actinoplanes sp. 50/110
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 76000, SDS-PAGE
Actinoplanes sp.
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Actinoplanes sp.
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
thermostable up to 40°C
Actinoplanes sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.2
6.9
-
Actinoplanes sp.
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Actinoplanes sp.
isoelectric focusing, pH gradient from 3 to 10
-
5
Other publictions for EC 2.4.99.B5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
729196
Leemhuis
Single amino acid mutations in ...
Actinoplanes sp.
Biochemistry
43
13204-13213
2004
-
-
-
-
8
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
729911
Hemker
Identification, cloning, expre ...
Actinoplanes sp., Actinoplanes sp. 50/110
J. Bacteriol.
183
4484-4492
2001
-
-
1
-
-
-
1
2
1
1
1
2
-
5
-
-
1
-
-
-
-
-
19
1
1
-
1
-
1
-
-
-
-
1
-
-
-
1
-
-
-
-
-
1
-
2
1
1
1
2
-
-
-
1
-
-
-
-
19
1
1
-
1
-
1
-
-
1
-
-
-
-
-
-