BRENDA - Enzyme Database
show all sequences of 2.4.99.21

Identification of genes involved in the biosynthesis and attachment of Methanococcus voltae N-linked glycans: insight into N-linked glycosylation pathways in Archaea

Chaban, B.; Voisin, S.; Kelly, J.; Logan, S.M.; Jarrell, K.F.; Mol. Microbiol. 61, 259-268 (2006)

Data extracted from this reference:

Organism
Organism
UniProt
Commentary
Textmining
Methanococcus voltae
Q2EMT4
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-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
the enzyme (AglB) has the ability to attach incomplete glycans as seen by the disaccharides present on flagellins and S-layer proteins in Methanococcus voltae aglA mutant cells. AglA is the glycosyl transferase involved in the attachment of the terminal sugar to the glycan
732528
Methanococcus voltae
?
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Synonyms
Synonyms
Commentary
Organism
AglB
-
Methanococcus voltae
Mv1749
-
Methanococcus voltae
MVO1749
locus name
Methanococcus voltae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
the enzyme (AglB) has the ability to attach incomplete glycans as seen by the disaccharides present on flagellins and S-layer proteins in Methanococcus voltae aglA mutant cells. AglA is the glycosyl transferase involved in the attachment of the terminal sugar to the glycan
732528
Methanococcus voltae
?
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-
General Information
General Information
Commentary
Organism
malfunction
wild-type cells are motile, mutant cells (with an insertional inactivation of the aglB gene) are non-motile. The mutant cells have detectable shifts in flagellin molecular weight. Thea are non-flaggelated, since only N-terminally processed flagellins can be assembled into flagella filaments
Methanococcus voltae
metabolism
the enzyme is involved in the transfer of the complete glycan to the flagellin and S-layer proteins
Methanococcus voltae
General Information (protein specific)
General Information
Commentary
Organism
malfunction
wild-type cells are motile, mutant cells (with an insertional inactivation of the aglB gene) are non-motile. The mutant cells have detectable shifts in flagellin molecular weight. Thea are non-flaggelated, since only N-terminally processed flagellins can be assembled into flagella filaments
Methanococcus voltae
metabolism
the enzyme is involved in the transfer of the complete glycan to the flagellin and S-layer proteins
Methanococcus voltae
Other publictions for EC 2.4.99.21
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738680
Esquivel
Identification of Haloferax vo ...
Haloferax volcanii
J. Biol. Chem.
291
10602-10614
2016
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738681
Taguchi
Comparative analysis of archae ...
Archaeoglobus fulgidus
J. Biol. Chem.
291
11042-11054
2016
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733612
Fujinami
Structural elucidation of an a ...
Archaeoglobus fulgidus
Carbohydr. Res.
413
55-62
2015
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726749
Cohen-Rosenzweig
Substrate promiscuity: AglB, t ...
Haloarcula marismortui, Halobacterium salinarum, Halobacterium salinarum DSM 671, Haloferax mediterranei
Appl. Environ. Microbiol.
80
486-496
2014
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3
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734650
Meyer
AglB, catalyzing the oligosacc ...
Sulfolobus acidocaldarius, Sulfolobus acidocaldarius DSM 639
MicrobiologyOpen
3
531-543
2014
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732558
Larkin
Biochemical evidence for an al ...
Methanococcus voltae
Nat. Chem. Biol.
9
367-373
2013
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735172
Matsumoto
Crystal structures of an archa ...
Archaeoglobus fulgidus
Proc. Natl. Acad. Sci. USA
110
17868-17873
2013
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734470
Abu-Qarn
Haloferax volcanii AglB and Ag ...
Haloferax volcanii, Haloferax volcanii DSM 3757
J. Mol. Biol.
374
1224-1236
2007
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732528
Chaban
Identification of genes involv ...
Methanococcus voltae
Mol. Microbiol.
61
259-268
2006
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