BRENDA - Enzyme Database
show all sequences of 2.4.99.20

CD38 is the major enzyme responsible for synthesis of nicotinic acid-adenine dinucleotide phosphate in mammalian tissues

Chini, E.N.; Chini, C.C.; Kato, I.; Takasawa, S.; Okamoto, H.; Biochem. J. 362, 125-130 (2002)

Data extracted from this reference:

Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
0.13 nM/min*mg
Mus musculus
-
-
mitochondrion
0.2 nM/min*mg
Mus musculus
5739
-
nucleus
0.4 nM/min*mg
Mus musculus
5634
-
plasma membrane
1.6 nM/min*mg
Mus musculus
5886
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
NADP+ + nicotinate
Rattus norvegicus
this multifunctional enzyme catalyses both the removal of nicotinamide from NADP+, forming 2'-phospho-cyclic ADP-ribose, and the addition of nicotinate to the cyclic product, forming nicotinic acid-adenine dinucleotide phosphate, a calcium messenger that can mobilize intracellular Ca2+ stores and activate Ca2+ influx to regulate a wide range of physiological processes
nicotinate-adenine dinucleotide phosphate + nicotinamide
-
-
?
NADP+ + nicotinate
Mus musculus
this multiunctional enzyme catalyses both the removal of nicotinamide from NADP+, forming 2'-phospho-cyclic ADP-ribose, and the addition of nicotinate to the cyclic product, forming nicotinic acid-adenine dinucleotide phosphate, a calcium messenger that can mobilize intracellular Ca2+ stores and activate Ca2+ influx to regulate a wide range of physiological processes
nicotinate-adenine dinucleotide phosphate + nicotinamide
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Mus musculus
-
-
-
Rattus norvegicus
Q64244
-
-
Reaction
Reaction
Commentary
Organism
Reaction ID
2'-phospho-cyclic ADP-ribose + nicotinate = nicotinate-adenine dinucleotide phosphate
reaction 1 b
Rattus norvegicus
NADP+ + nicotinate = nicotinate-adenine dinucleotide phosphate + nicotinamide
overall reaction. The multifunctional enzyme catalyses both the removal of nicotinamide from NADP+, forming 2'-phospho-cyclic ADP-ribose, and the addition of nicotinate to the cyclic product, forming nicotinic acid-adenine dinucleotide phosphate, a calcium messenger that can mobilize intracellular Ca2+ stores and activate Ca2+ influx to regulate a wide range of physiological processes
Rattus norvegicus
NADP+ = 2'-phospho-cyclic ADP-ribose + nicotinamide
reaction 1a
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
-
Mus musculus
-
brain
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-
lung
-
Rattus norvegicus
-
additional information
very low activity in kidney cortex and skeletal muscles
Rattus norvegicus
-
myocardium
-
Rattus norvegicus
-
spleen
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00000013
-
37°C, pH 7.2, microsome from CD38(+/+) wild-type mouse brain
Mus musculus
0.0000002
-
37°C, pH 7.2, mitochondria from CD38(+/+) wild-type mouse brain
Mus musculus
0.0000016
-
37°C, pH 7.2, plasma membrane from CD38(+/+) wild-type mouse brain
Mus musculus
0.4
-
37°C, pH 7.2, nucleus from CD38(+/+) wild-type mouse brain
Mus musculus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2'-phospho-cyclic ADP-ribose + nicotinate
-
724210
Mus musculus
nicotinate-adenine dinucleotide phosphate
-
-
-
?
2'-phospho-cyclic ADP-ribose + nicotinate
-
724210
Rattus norvegicus
nicotinate-adenine dinucleotide phosphate
-
-
-
?
NADP+ + nicotinate
-
724210
Mus musculus
nicotinate-adenine dinucleotide phosphate + nicotinamide
nicotinic acid-adenine dinucleotide phosphate i.e. NAADP+
-
-
?
NADP+ + nicotinate
-
724210
Rattus norvegicus
nicotinate-adenine dinucleotide phosphate + nicotinamide
nicotinic acid-adenine dinucleotide phosphate i.e. NAADP+
-
-
?
NADP+ + nicotinate
this multifunctional enzyme catalyses both the removal of nicotinamide from NADP+, forming 2'-phospho-cyclic ADP-ribose, and the addition of nicotinate to the cyclic product, forming nicotinic acid-adenine dinucleotide phosphate, a calcium messenger that can mobilize intracellular Ca2+ stores and activate Ca2+ influx to regulate a wide range of physiological processes
724210
Rattus norvegicus
nicotinate-adenine dinucleotide phosphate + nicotinamide
-
-
-
?
NADP+ + nicotinate
this multiunctional enzyme catalyses both the removal of nicotinamide from NADP+, forming 2'-phospho-cyclic ADP-ribose, and the addition of nicotinate to the cyclic product, forming nicotinic acid-adenine dinucleotide phosphate, a calcium messenger that can mobilize intracellular Ca2+ stores and activate Ca2+ influx to regulate a wide range of physiological processes
724210
Mus musculus
nicotinate-adenine dinucleotide phosphate + nicotinamide
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
BST1
-
Mus musculus
BST1
-
Rattus norvegicus
CD38
-
Mus musculus
CD38
-
Rattus norvegicus
diphosphopyridine nucleosidase
ambiguous
Mus musculus
diphosphopyridine nucleosidase
ambiguous
Rattus norvegicus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Mus musculus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
assay at
Mus musculus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
0.13 nM/min*mg
Mus musculus
-
-
mitochondrion
0.2 nM/min*mg
Mus musculus
5739
-
nucleus
0.4 nM/min*mg
Mus musculus
5634
-
plasma membrane
1.6 nM/min*mg
Mus musculus
5886
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
NADP+ + nicotinate
Rattus norvegicus
this multifunctional enzyme catalyses both the removal of nicotinamide from NADP+, forming 2'-phospho-cyclic ADP-ribose, and the addition of nicotinate to the cyclic product, forming nicotinic acid-adenine dinucleotide phosphate, a calcium messenger that can mobilize intracellular Ca2+ stores and activate Ca2+ influx to regulate a wide range of physiological processes
nicotinate-adenine dinucleotide phosphate + nicotinamide
-
-
?
NADP+ + nicotinate
Mus musculus
this multiunctional enzyme catalyses both the removal of nicotinamide from NADP+, forming 2'-phospho-cyclic ADP-ribose, and the addition of nicotinate to the cyclic product, forming nicotinic acid-adenine dinucleotide phosphate, a calcium messenger that can mobilize intracellular Ca2+ stores and activate Ca2+ influx to regulate a wide range of physiological processes
nicotinate-adenine dinucleotide phosphate + nicotinamide
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
-
Mus musculus
-
brain
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-
lung
-
Rattus norvegicus
-
additional information
very low activity in kidney cortex and skeletal muscles
Rattus norvegicus
-
myocardium
-
Rattus norvegicus
-
spleen
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00000013
-
37°C, pH 7.2, microsome from CD38(+/+) wild-type mouse brain
Mus musculus
0.0000002
-
37°C, pH 7.2, mitochondria from CD38(+/+) wild-type mouse brain
Mus musculus
0.0000016
-
37°C, pH 7.2, plasma membrane from CD38(+/+) wild-type mouse brain
Mus musculus
0.4
-
37°C, pH 7.2, nucleus from CD38(+/+) wild-type mouse brain
Mus musculus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2'-phospho-cyclic ADP-ribose + nicotinate
-
724210
Mus musculus
nicotinate-adenine dinucleotide phosphate
-
-
-
?
2'-phospho-cyclic ADP-ribose + nicotinate
-
724210
Rattus norvegicus
nicotinate-adenine dinucleotide phosphate
-
-
-
?
NADP+ + nicotinate
-
724210
Mus musculus
nicotinate-adenine dinucleotide phosphate + nicotinamide
nicotinic acid-adenine dinucleotide phosphate i.e. NAADP+
-
-
?
NADP+ + nicotinate
-
724210
Rattus norvegicus
nicotinate-adenine dinucleotide phosphate + nicotinamide
nicotinic acid-adenine dinucleotide phosphate i.e. NAADP+
-
-
?
NADP+ + nicotinate
this multifunctional enzyme catalyses both the removal of nicotinamide from NADP+, forming 2'-phospho-cyclic ADP-ribose, and the addition of nicotinate to the cyclic product, forming nicotinic acid-adenine dinucleotide phosphate, a calcium messenger that can mobilize intracellular Ca2+ stores and activate Ca2+ influx to regulate a wide range of physiological processes
724210
Rattus norvegicus
nicotinate-adenine dinucleotide phosphate + nicotinamide
-
-
-
?
NADP+ + nicotinate
this multiunctional enzyme catalyses both the removal of nicotinamide from NADP+, forming 2'-phospho-cyclic ADP-ribose, and the addition of nicotinate to the cyclic product, forming nicotinic acid-adenine dinucleotide phosphate, a calcium messenger that can mobilize intracellular Ca2+ stores and activate Ca2+ influx to regulate a wide range of physiological processes
724210
Mus musculus
nicotinate-adenine dinucleotide phosphate + nicotinamide
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Mus musculus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
assay at
Mus musculus
General Information
General Information
Commentary
Organism
malfunction
generation of CD38(-/-) knockout mice of determine the role of this enzyme
Mus musculus
physiological function
the enzyme is involved in synthesis of nicotinic acid-adenine dinucleotide phosphate, a calcium messenger that can mobilize intracellular Ca2+ stores and activate Ca2+ influx to regulate a wide range of physiological processes
Mus musculus
physiological function
the enzyme is involved in synthesis of nicotinic acid-adenine dinucleotide phosphate, a calcium messenger that can mobilize intracellular Ca2+ stores and activate Ca2+ influx to regulate a wide range of physiological processes
Rattus norvegicus
General Information (protein specific)
General Information
Commentary
Organism
malfunction
generation of CD38(-/-) knockout mice of determine the role of this enzyme
Mus musculus
physiological function
the enzyme is involved in synthesis of nicotinic acid-adenine dinucleotide phosphate, a calcium messenger that can mobilize intracellular Ca2+ stores and activate Ca2+ influx to regulate a wide range of physiological processes
Mus musculus
physiological function
the enzyme is involved in synthesis of nicotinic acid-adenine dinucleotide phosphate, a calcium messenger that can mobilize intracellular Ca2+ stores and activate Ca2+ influx to regulate a wide range of physiological processes
Rattus norvegicus
Other publictions for EC 2.4.99.20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
727515
Schmid
CD38: a NAADP degrading enzyme ...
Homo sapiens, Mus musculus
FEBS Lett.
585
3544-3548
2011
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2
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1
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-
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2
2
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-
-
727868
Rah
Generation of cyclic ADP-ribos ...
Mus musculus
J. Biol. Chem.
285
21877-21887
2010
-
-
-
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-
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-
1
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1
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1
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1
1
-
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-
726853
Moreschi
NAADP+ synthesis from cADPRP a ...
Aplysia californica, Axinella polypoides, Homo sapiens
Biochem. Biophys. Res. Commun.
345
573-580
2006
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-
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3
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9
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9
-
-
-
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-
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-
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727857
Graeff
Acidic residues at the active ...
Homo sapiens
J. Biol. Chem.
281
28951-28957
2006
-
-
1
1
5
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1
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1
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1
5
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726898
Bacher
Channelling of substrate promi ...
Oryctolagus cuniculus
Biochem. J.
381
147-154
2004
-
-
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6
1
2
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1
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4
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5
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5
6
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1
2
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4
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-
-
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-
-
-
-
-
724210
Chini
CD38 is the major enzyme respo ...
Mus musculus, Rattus norvegicus
Biochem. J.
362
125-130
2002
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-
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4
-
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2
-
2
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3
-
7
4
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6
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6
1
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1
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4
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2
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7
4
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6
-
1
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1
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3
3
-
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-
726647
Lee
ADP-ribosyl cyclase and CD38. ...
Aplysia californica, Homo sapiens
Adv. Exp. Med. Biol.
419
411-419
1997
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1
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2
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8
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6
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1
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8
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727816
Aarhus
ADP-ribosyl cyclase and CD38 c ...
Aplysia californica, Homo sapiens
J. Biol. Chem.
270
30327-30333
1995
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2
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2
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6
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6
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