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Literature summary for 2.4.99.18 extracted from

  • Chen, M.M.; Glover, K.J.; Imperiali, B.
    From peptide to protein: comparative analysis of the substrate specificity of N-linked glycosylation in C. jejuni (2007), Biochemistry, 46, 5579-5585.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0008
-
acetyl-DQNAT-(4-nitrophenylalanine)-NH2
-
Campylobacter jejuni
0.00087
-
acetyl-DFNAT-(4-nitrophenylalanine)-NH2
-
Campylobacter jejuni
0.00106
-
acetyl-DVNAT-(4-nitrophenylalanine)-NH2
-
Campylobacter jejuni
0.00122
-
acetyl-DFNVT-(4-nitrophenylalanine)-NH2
-
Campylobacter jejuni
0.00144
-
acetyl-DVNVT-(4-nitrophenylalanine)-NH2
-
Campylobacter jejuni
0.003
-
acetyl-DVNAS-(4-nitrophenylalanine)-NH2
-
Campylobacter jejuni
0.0225
-
acetyl-EVNAT-(4-nitrophenylalanine)-NH2
-
Campylobacter jejuni

Organism

Organism UniProt Comment Textmining
Campylobacter jejuni
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dolichyl diphosphooligosaccharide + protein L-asparagine DQNAT is the optimal acceptor substrate. PglB is not capable of utilizing glycosyl donors such as dolichyl-pyrophosphatechitobiose Campylobacter jejuni dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine
-
?
GalNAc-N,N'-diacetyl-bacillosamine-PP-undecaprenyl + acetyl-DFNAT-(4-nitrophenylalanine)-NH2
-
Campylobacter jejuni ?
-
?
GalNAc-N,N'-diacetyl-bacillosamine-PP-undecaprenyl + acetyl-DFNVT-(4-nitrophenylalanine)-NH2
-
Campylobacter jejuni ?
-
?
GalNAc-N,N'-diacetyl-bacillosamine-PP-undecaprenyl + acetyl-DQNAT-(4-nitrophenylalanine)-NH2
-
Campylobacter jejuni ?
-
?
GalNAc-N,N'-diacetyl-bacillosamine-PP-undecaprenyl + acetyl-DVNAS-(4-nitrophenylalanine)-NH2
-
Campylobacter jejuni ?
-
?
GalNAc-N,N'-diacetyl-bacillosamine-PP-undecaprenyl + acetyl-DVNAT-(4-nitrophenylalanine)-NH2
-
Campylobacter jejuni ?
-
?
GalNAc-N,N'-diacetyl-bacillosamine-PP-undecaprenyl + acetyl-DVNVT-(4-nitrophenylalanine)-NH2
-
Campylobacter jejuni ?
-
?
GalNAc-N,N'-diacetyl-bacillosamine-PP-undecaprenyl + acetyl-EVNAT-(4-nitrophenylalanine)-NH2
-
Campylobacter jejuni ?
-
?
additional information no activity with acetyl-NVAAT-(para-nitrophenylalanine)-NH2 and acetyl-DVAAT-(para-nitrophenylalanine)-NH2 Campylobacter jejuni ?
-
?

Synonyms

Synonyms Comment Organism
PglB
-
Campylobacter jejuni