Literature summary for 2.4.99.17 extracted from

Mathews, I.; Schwarzenbacher, R.; McMullan, D.; Abdubek, P.; Ambing, E.; Axelrod, H.; Biorac, T.; Canaves, J.; Chiu, H.; Deacon, A.; DiDonato, M.; Elsliger, M.; Godzik, A.; Grittini, C.; Grzechnik, S.; Hale, J.; Hampton, E.; Han, G.; Haugen, J.; Hornsby,
Crystal structure of S-adenosylmethionine:tRNA ribosyltransferase-isomerase (QueA) from Thermotoga maritima at 2.0 A resolution reveals a new fold (2005), Proteins, 59, 869-874.

Search for more literature for 2.4.99.17

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli strain DL41	Thermotoga maritima

Crystallization (Commentary)

Crystallization (Comment)	Organism
nanodroplet vapor diffusion method, using 12% (w/v) polyethylene glycol 4000, 0.1 M citric acid at pH 5.0	Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
38529	-	x * 38529, calculated from amino acid sequence	Thermotoga maritima

Organism

Organism	UniProt	Comment	Textmining
Thermotoga maritima	Q9WZ44	-	-

Purification (Commentary)

Purification (Comment)	Organism
nickel-resin column chromatography, RESOURCE Q column chromatography, and Superdex 200 gel filtration	Thermotoga maritima

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + 7-aminomethyl-7-deazaguanine34 in tRNA	the enzyme catalyzes the formation of the 2,3-epoxy-4,5-dihydroxycyclopentane ring of the queuosine precursor epoxyqueuosine	Thermotoga maritima	L-methionine + adenine + epoxyqueuosine34 in tRNA	-	?

Subunits

Subunits	Comment	Organism
?	x * 38529, calculated from amino acid sequence	Thermotoga maritima

Synonyms

Synonyms	Comment	Organism
QueA	-	Thermotoga maritima
queuosine biosynthesis protein	-	Thermotoga maritima
S-adenosylmethionine:tRNA ribosyltransferase-isomerase	-	Thermotoga maritima
TM0574	-	Thermotoga maritima

pI Value

Organism	Comment	pI Value Maximum	pI Value
Thermotoga maritima	calculated from amino acid sequence	-	8.6

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)