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Literature summary for 2.4.2.9 extracted from

  • Leonhardt, N.; Divol, F.; Chiarenza, S.; Deschamps, S.; Renaud, J.; Giacalone, C.; Laurent, N.; Berthome, R.; Peret, B.
    Tissue-specific inactivation by cytosine deaminase/uracil phosphoribosyl transferase as a tool to study plant biology (2019), Plant J., 101, 731-741 .
    View publication on PubMed

Application

Application Comment Organism
molecular biology engineering the yeast fluorocytosine deaminase (FCY1) gene by creating a fusion with the bacterial uracil phosphoribosyl transferase (UPP) gene results in a recombinant protein that converts the precursor 5-fluorocytosine (5-FC) into 5-fluorouracyl, a drug used in the treatment of a range of cancers, which triggers DNA and RNA damage. The tissue-specific FCY-UPP system is a great tool to inactivate cells in a precise spatial and temporal manner, method evaluation, overview Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
gene upp, recombinant overexpression of 35S:FCY-UPP and pMYB60:FCY-UPP constructs in Arabdopsis thaliana ecotype Col-0, which affects the effect of 5-fluorouracil (5-FC) on the plants compared to wild-type plants, analysis of the tissue specificity of the FCY-UPP tandem, overview. The enzyme tandem is expressed in the xylem pole pericycle cells from which lateral roots are being formed. Expressing FCYUPP in the lateral root cap and epidermis using the J0951 enhancer trap line results in a strong reduction of primary root growth. Similarly, when FCY-UPP proteins are expressed in the guard cells using the pMYB60 promoter, no reduction in total leaf area is observed. These observations suggest that both the FCY-UPP tandem protein and the products of 5-FC incorporation remain in the cells where the construct is expressed, at least on a short time-scale. A tagged version of FCY-UPP fused to the GFP reporter is expressed under the epidermis-specific promoter of WEREWOLF Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information engineering the yeast fluorocytosine deaminase (FCY1) gene by creating a fusion with the bacterial uracil phosphoribosyl transferase (UPP) gene results in a recombinant protein that converts the precursor 5-fluorocytosine (5-FC) into 5-fluorouracyl, a drug used in the treatment of a range of cancers, which triggers DNA and RNA damage. The FCY-UPP gene construct is expressed in specific cell types using enhancer trap lines and promoters, demonstrating that this marker acts in a cell-autonomous manner. It can inactivate slow developmental processes like lateral root formation by targeting pericycle cells. A role for the lateral root cap and the epidermis in controlling root growth, a faster response, is also revealed. The 5-FC precursor acts systemically, as demonstrated by its ability to inhibit stomatal movements when supplied to the roots in combination with a guard cell-specific promoter. The tissular inactivation is reversible, and can therefore be used to synchronize plant responses or to determine cell type-specific functions during different developmental stages Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UMP + diphosphate Escherichia coli
-
uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A8F0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UMP + diphosphate
-
Escherichia coli uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
?

Synonyms

Synonyms Comment Organism
UPP
-
Escherichia coli
uracil phosphoribosyl transferase
-
Escherichia coli