Any feedback?
Literature summary for 2.4.2.9 extracted from
- Substrate inhibition of uracil phosphoribosyltransferase by uracil can account for the uracil growth sensitivity of Leishmania donovani pyrimidine auxotrophs (2013), J. Biol. Chem., 288, 29954-29964.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21Star (DE3) One Shot cells | Leishmania donovani |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-thiouracil | substrate inhibition at high concentrations | Leishmania donovani |  |
| 5-fluorouracil | substrate inhibition at high concentrations | Leishmania donovani | |
| EDTA | - |
Leishmania donovani | |
| Uracil | substrate inhibition at high concentrations | Leishmania donovani | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0064 | - |
5-fluorouracil | at pH 7.5 and 25°C | Leishmania donovani | |
| 0.0071 | - |
4-thiouracil | at pH 7.5 and 25°C | Leishmania donovani | |
| 0.0204 | - |
Uracil | at pH 7.5 and 25°C | Leishmania donovani | |
| 0.0993 | - |
5-phospho-alpha-D-ribose 1-diphosphate | with uracil as cosubstrate, at pH 7.5 and 25°C | Leishmania donovani | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | can replace Mg2+ | Leishmania donovani | |
| Mg2+ | maximum activity in the presence of 5 mM Mg2+ | Leishmania donovani | |
| Mn2+ | can replace Mg2+ | Leishmania donovani | |
| additional information | not stimulated by Ba2+, Ca2+, Ni2+, and Zn2+ | Leishmania donovani |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
4 * 27000, SDS-PAGE | Leishmania donovani |
| 100000 | - |
gel filtration | Leishmania donovani |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| uracil + 5-phospho-alpha-D-ribose 1-diphosphate | Leishmania donovani | - |
UMP + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leishmania donovani | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Leishmania donovani |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-thiouracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Leishmania donovani | 4-thio-UMP + diphosphate | - |
? | |
| 5-fluorouracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Leishmania donovani | 5-fluoro-UMP + diphosphate | - |
? | |
| uracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Leishmania donovani | UMP + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 27000, SDS-PAGE | Leishmania donovani |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UPRT | - |
Leishmania donovani |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Leishmania donovani |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | more than 90% activity between 7.5 and 8.5 with sharp drops in activity 0.5 pH units outside this range | Leishmania donovani |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
