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Literature summary for 2.4.2.9 extracted from

  • Linde, L.; Jensen, K.F.
    Uracil phosphoribosyltransferase from the extreme thermoacidophilic archaebacterium Sulfolobus shibatae is an allosteric enzyme, activated by GTP and inhibited by CTP. (1996), Biochim. Biophys. Acta, 1296, 16-22.
    View publication on PubMed
Search for more literature for 2.4.2.9

Activating Compound

Activating Compound Comment Organism Structure
ATP weak activation Saccharolobus shibatae
GTP strong activation at pH 6 and at pH 8. The concentration of GTP required for half-maximal activation is about 0.080 mM Saccharolobus shibatae

Inhibitors

Inhibitors Comment Organism Structure
CTP
-
 Saccharolobus shibatae
UMP
-
 Saccharolobus shibatae
UTP
-
 Saccharolobus shibatae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 Uracil pH 6.1, without GTP Saccharolobus shibatae
0.002
-
 Uracil pH 8.0, without GTP Saccharolobus shibatae
0.004
-
 Uracil pH 6.1, presence of 1 mM GTP Saccharolobus shibatae
0.005
-
 Uracil pH 8.0, presence of 1 mM GTP Saccharolobus shibatae
0.016
-
 5-phospho-alpha-D-ribose 1-diphosphate pH 8.0, without GTP Saccharolobus shibatae
0.049
-
 5-phospho-alpha-D-ribose 1-diphosphate pH 8.0, presence of 1 mM GTP Saccharolobus shibatae
0.079
-
 5-phospho-alpha-D-ribose 1-diphosphate pH 6.1, without GTP Saccharolobus shibatae
0.115
-
 5-phospho-alpha-D-ribose 1-diphosphate pH 6.1, presence of 1 mM GTP Saccharolobus shibatae

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ UPRTase can use Mn2+, Co2+ and Zn2+ instead of Mg2+. While the saturation curve for Mg2+ is smoothly increasing, these three metal ions are inhibitory at higher concentrations Saccharolobus shibatae
Mg2+ absolute requirement for Mg2+ Saccharolobus shibatae
Mn2+ UPRTase can use Mn2+, Co2+ and Zn2+ instead of Mg2+. While the saturation curve for Mg2+ is smoothly increasing, these three metal ions are inhibitory at higher concentrations Saccharolobus shibatae
Zn2+ UPRTase can use Mn2+, Co2+ and Zn2+ instead of Mg2+. While the saturation curve for Mg2+ is smoothly increasing, these three metal ions are inhibitory at higher concentrations Saccharolobus shibatae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
200000 250000 gel filtration Saccharolobus shibatae

Organism

Organism UniProt Comment Textmining
Saccharolobus shibatae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Saccharolobus shibatae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.138
-
-
 Saccharolobus shibatae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Saccharolobus shibatae UMP + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
UPRTase
-
 Saccharolobus shibatae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
 optimal temperature exceeds 95°C Saccharolobus shibatae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 stable for at least 1 h Saccharolobus shibatae
90
-
 half-life: 4 min Saccharolobus shibatae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4
-
-
 Saccharolobus shibatae

pH Range

pH Minimum pH Maximum Comment Organism
5.8 7 pH 5.8: about 55% of maximal activity, pH 7.0: about 45% of maximal activity Saccharolobus shibatae