Literature summary for 2.4.2.9 extracted from
Barchue, J.; Symersky, J.; Narayana, S.V.; Moore, J.K.; DeLucas, L.J.; Chattopadhyay, D.
Expression, purification, crystallization and preliminary X-ray diffraction analysis of uracil phosphoribosyltransferase of Toxoplasma gondii (1999), Acta Crystallogr. Sect. D, 55, 347-349.
No PubMed abstract available
Cloned(Commentary)
Cloned (Comment) |
Organism |
DNA sequence determination, functional overexpression in Escherichia coli BL21(DE3)plyS |
Toxoplasma gondii |
Crystallization (Commentary)
Crystallization (Comment) |
Organism |
hanging drop-vapour diffusion technique, 2.0 M ammonium phosphate as precipitant, pH 8.0, room temperature, X-ray diffraction structure analysis, crystals are enzymatically active, wild-type and mutant enzymes |
Toxoplasma gondii |
Molecular Weight [Da]
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
28000 |
- |
x * 28000, recombinant protein, SDS-PAGE |
Toxoplasma gondii |
Organism
Organism |
UniProt |
Comment |
Textmining |
Toxoplasma gondii |
Q26998 |
- |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
recombinant from Escherichia coli |
Toxoplasma gondii |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
uracil + 5-phospho-alpha-D-ribose 1-diphosphate |
- |
Toxoplasma gondii |
UMP + diphosphate |
- |
? |
|
Subunits
Subunits |
Comment |
Organism |
? |
x * 28000, recombinant protein, SDS-PAGE |
Toxoplasma gondii |
pH Optimum
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
7.5 |
8 |
- |
Toxoplasma gondii |