BRENDA - Enzyme Database
show all sequences of 2.4.2.9

Bakers yeast UMP:pyrophosphate phosphoribosyltransferase. Purification, enzymatic and kinetic properties

Natalini, P.; Ruggieri, S.; Santarelli, I.; Vita, A.; Magni, G.; J. Biol. Chem. 254, 1558-1563 (1979)

Data extracted from this reference:

General Stability
General Stability
Organism
enzyme is highly unstable, dimethyl sulfoxide stabilizes during purification
Saccharomyces cerevisiae
Inhibitors
Inhibitors
Commentary
Organism
Structure
dCMP
allosteric inhibition
Saccharomyces cerevisiae
dCTP
allosteric inhibition
Saccharomyces cerevisiae
dUMP
allosteric inhibition
Saccharomyces cerevisiae
TTP
allosteric inhibition
Saccharomyces cerevisiae
UDP
allosteric inhibition
Saccharomyces cerevisiae
UMP
allosteric inhibition
Saccharomyces cerevisiae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.021
-
Uracil
-
Saccharomyces cerevisiae
0.026
-
5-phospho-alpha-D-ribose 1-diphosphate
-
Saccharomyces cerevisiae
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Saccharomyces cerevisiae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
80000
-
gel filtration
Saccharomyces cerevisiae
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
Commentary
Organism
-
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
638365
Saccharomyces cerevisiae
UMP + diphosphate
-
638365
Saccharomyces cerevisiae
?
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
22
42
-
Saccharomyces cerevisiae
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
60
-
2 min, complete loss of activity, 1 mM uracil + 0.1 EDTA protect partially
Saccharomyces cerevisiae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.6
7.8
-
Saccharomyces cerevisiae
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.5
10
-
Saccharomyces cerevisiae
General Stability (protein specific)
General Stability
Organism
enzyme is highly unstable, dimethyl sulfoxide stabilizes during purification
Saccharomyces cerevisiae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
dCMP
allosteric inhibition
Saccharomyces cerevisiae
dCTP
allosteric inhibition
Saccharomyces cerevisiae
dUMP
allosteric inhibition
Saccharomyces cerevisiae
TTP
allosteric inhibition
Saccharomyces cerevisiae
UDP
allosteric inhibition
Saccharomyces cerevisiae
UMP
allosteric inhibition
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.021
-
Uracil
-
Saccharomyces cerevisiae
0.026
-
5-phospho-alpha-D-ribose 1-diphosphate
-
Saccharomyces cerevisiae
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
80000
-
gel filtration
Saccharomyces cerevisiae
Purification (Commentary) (protein specific)
Commentary
Organism
-
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
638365
Saccharomyces cerevisiae
UMP + diphosphate
-
638365
Saccharomyces cerevisiae
?
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
22
42
-
Saccharomyces cerevisiae
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
60
-
2 min, complete loss of activity, 1 mM uracil + 0.1 EDTA protect partially
Saccharomyces cerevisiae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.6
7.8
-
Saccharomyces cerevisiae
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.5
10
-
Saccharomyces cerevisiae
Other publictions for EC 2.4.2.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736257
Narayanan
Unravelling the potential of a ...
Arabidopsis thaliana
Int. J. Biol. Macromol.
91
310-316
2016
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735615
Ghode
Structural basis of mapping th ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Biochem. Biophys. Res. Commun.
467
577-582
2015
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1
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1
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736258
Hasegawa
Cyclophosphamide enhances anti ...
Syrian hamster adenovirus
Int. J. Cancer
133
1479-1488
2013
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1
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6
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1
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736431
Soysa
Substrate inhibition of uracil ...
Leishmania donovani
J. Biol. Chem.
288
29954-29964
2013
-
-
1
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4
4
-
4
2
1
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3
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1
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1
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1
1
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4
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2
1
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3
1
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1
1
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737104
Villela
Biochemical characterization o ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
PLoS ONE
8
e56445
2013
-
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1
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2
1
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1
3
2
-
161
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1
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1
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2
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1
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2
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2
1
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1
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722407
Altanerova
Human adipose tissue-derived m ...
Saccharomyces cerevisiae
Int. J. Cancer
130
2455-2463
2012
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1
1
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1
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723085
Yata
-
Interaction studies of E. coli ...
Escherichia coli
Med. Chem. Res.
21
1149-1155
2012
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1
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1
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1
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1
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1
1
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703007
Tanaka
Bystander effect from cytosine ...
Homo sapiens
Cancer Lett.
282
43-47
2009
-
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1
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-
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-
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-
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1
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705159
Christoffersen
Structural and kinetic studies ...
Saccharolobus solfataricus
J. Mol. Biol.
393
464-477
2009
1
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1
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1
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706237
Mainguet
Uracil salvage is necessary fo ...
Arabidopsis thaliana
Plant J.
60
280-291
2009
-
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1
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1
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1
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4
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2
2
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684230
Xing
Non-invasive molecular and fun ...
Homo sapiens
Acta Oncol.
47
1211-1220
2008
-
1
1
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1
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685041
Perez-Zuniga
Bisphosphonates activate the 5 ...
Saccharomyces cerevisiae
Biochem. Pharmacol.
76
825-830
2008
5
-
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1
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688833
Zeiner
RNA analysis by biosynthetic t ...
Toxoplasma gondii
Methods Mol. Biol.
419
135-146
2008
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2
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1
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688919
Gopinath
Apoptotic induction with bifun ...
Escherichia coli
Mol. Biotechnol.
39
39-48
2008
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1
1
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2
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689850
Zhao
Specific targeting of prostate ...
Escherichia coli
Prostate Cancer Prostatic. Dis.
12
166-171
2008
-
1
1
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2
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3
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2
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1
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675195
Li
Identification and characteriz ...
Homo sapiens
J. Hum. Genet.
52
415-422
2007
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2
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1
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9
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1
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10
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1
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4
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1
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2
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19
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2
1
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2
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676556
Islam
Functional characterization of ...
Arabidopsis thaliana
Plant Mol. Biol.
63
465-477
2007
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3
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5
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2
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2
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685910
Lu
Gene therapy for ovarian cance ...
Homo sapiens
Cancer Biol. Ther.
6
397-404
2007
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689317
Fogar
Suicide gene therapy with the ...
Saccharomyces cerevisiae
Pancreas
35
224-231
2007
-
1
1
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673932
O'Brien
Coexpression of the uracil pho ...
Saccharomyces cerevisiae
Hum. Gene Ther.
17
518-530
2006
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1
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1
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1
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3
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1
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1
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1
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674286
Arsene-Ploetze
Uracil salvage pathway in Lact ...
Lactobacillus plantarum, Lactobacillus plantarum BM604
J. Bacteriol.
188
4777-4786
2006
-
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1
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1
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4
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675179
Khatri
Combination of cytosine deamin ...
Mus musculus
J. Gene Med.
8
1086-1096
2006
-
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1
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1
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1
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3
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2
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2
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658133
Arent
Allosteric regulation and comm ...
Saccharolobus solfataricus
Biochemistry
44
883-892
2005
-
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