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Literature summary for 2.4.2.8 extracted from

  • Patta, P.; Martinelli, L.; Rotta, M.; Abbadi, B.; Santos, D.; Basso, L.
    Mode of action of recombinant hypoxanthine-guanine phosphoribosyltransferase from Mycobacterium tuberculosis (2015), RSC Adv., 5, 74671-74683.
No PubMed abstract available
Search for more literature for 2.4.2.8

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0065
-
 5-phospho-alpha-D-ribose 1-diphosphate with guanine as cosubstrate, at pH 7.5 and 25°C Mycobacterium tuberculosis
0.01
-
 guanine at pH 7.5 and 25°C Mycobacterium tuberculosis
0.0141
-
 5-phospho-alpha-D-ribose 1-diphosphate with hypoxanthine as cosubstrate, at pH 7.5 and 25°C Mycobacterium tuberculosis
0.026
-
 hypoxanthine at pH 7.5 and 25°C Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22251
-
 2 * 22251, calculated from amino acid sequence Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
guanine + 5-phospho-alpha-D-ribose 1-diphosphate Mycobacterium tuberculosis
-
 GMP + diphosphate
-
 ?
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate Mycobacterium tuberculosis
-
 IMP + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WHQ9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Mycobacterium tuberculosis GMP + diphosphate
-
 ?
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Mycobacterium tuberculosis IMP + diphosphate
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 22251, calculated from amino acid sequence Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
HGPRT
-
 Mycobacterium tuberculosis
hypoxanthine-guanine phosphoribosyltransferase
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.193
-
 guanine at pH 7.5 and 25°C Mycobacterium tuberculosis
0.89
-
 hypoxanthine at pH 7.5 and 25°C Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.29
-
 5-phospho-alpha-D-ribose 1-diphosphate with guanine as cosubstrate, at pH 7.5 and 25°C Mycobacterium tuberculosis
0.63
-
 5-phospho-alpha-D-ribose 1-diphosphate with hypoxanthine as cosubstrate, at pH 7.5 and 25°C Mycobacterium tuberculosis
19
-
 guanine at pH 7.5 and 25°C Mycobacterium tuberculosis
34
-
 hypoxanthine at pH 7.5 and 25°C Mycobacterium tuberculosis