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Literature summary for 2.4.2.8 extracted from

  • Naesens, L.; Guddat, L.W.; Keough, D.T.; van Kuilenburg, A.B.; Meijer, J.; Vande Voorde, J.; Balzarini, J.
    Role of human hypoxanthine guanine phosphoribosyltransferase in activation of the antiviral agent T-705 (favipiravir) (2013), Mol. Pharmacol., 84, 615-629.
    View publication on PubMed
Search for more literature for 2.4.2.8

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with T-705-RMP, hanging drop vapor diffusion method, using 0.2 M NaCl, 1 M sodium/potassium tartrate, 0.1 M imidazole, pH 8.0 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0036
-
 hypoxanthine at pH 7.4 and 37°C Homo sapiens
0.0054
-
 guanine at pH 7.4 and 37°C Homo sapiens
4.069
-
 3-hydroxy-2-pyrazinecarboxamide at pH 7.4 and 37°C Homo sapiens
6.426
-
 6-fluoro-3-hydroxy-2-pyrazinecarboxamide at pH 7.4 and 37°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 x * 25000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P00492
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293T cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxy-2-pyrazinecarboxamide + 5-phospho-alpha-D-ribose 1-diphosphate T-1105, poor substrate Homo sapiens 3-oxo-4-(5-O-phosphono-beta-D-ribofuranosyl)-3,4-dihydropyrazine-2-carboxamide + diphosphate
-
 ?
3-hydroxy-2-pyrazinecarboxamide + 5-phospho-alpha-D-ribose 1-diphosphate T-1105, poor substrate Homo sapiens ? + diphosphate
-
 ?
6-fluoro-3-hydroxy-2-pyrazinecarboxamide + 5-phospho-alpha-D-ribose 1-diphosphate T-705, poor substrate Homo sapiens 6-fluoro-3-oxo-4-(5-O-phosphono-beta-D-ribofuranosyl)-3,4-dihydropyrazine-2-carboxamide + diphosphate
-
 ?
6-fluoro-3-hydroxy-2-pyrazinecarboxamide + 5-phospho-alpha-D-ribose 1-diphosphate T-705, poor substrate Homo sapiens ? + diphosphate
-
 ?
guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Homo sapiens GMP + diphosphate
-
 ?
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Homo sapiens IMP + diphosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 25000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
HGPRT
-
 Homo sapiens
hypoxanthine guanine phosphoribosyltransferase
-
 Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.027
-
 6-fluoro-3-hydroxy-2-pyrazinecarboxamide at pH 7.4 and 37°C Homo sapiens
0.14
-
 3-hydroxy-2-pyrazinecarboxamide at pH 7.4 and 37°C Homo sapiens
130
-
 hypoxanthine at pH 7.4 and 37°C Homo sapiens
140
-
 guanine at pH 7.4 and 37°C Homo sapiens