Crystallization (Comment) | Organism |
---|---|
apo-form and in complex with GMP, sitting drop vapor diffusion method, using 0.2 M trimethylamine N-oxide dehydrate, 0.1 M Tris pH 8.5, 20% (w/v) polyethylene glycol monomethyl ether 2000 | Legionella pneumophila |
Protein Variants | Comment | Organism |
---|---|---|
S103R | the mutation is associated with Gout arthritis and shows strongly reduced activity compared to the wild type enzyme | Legionella pneumophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0105 | - |
guanine | with guanine as cosubstrate, wild type enzyme, at pH 7.5 and 25°C | Legionella pneumophila | |
0.06 | - |
5-phospho-alpha-D-ribose 1-diphosphate | with guanine as cosubstrate, wild type enzyme, at pH 7.5 and 25°C | Legionella pneumophila | |
0.104 | - |
5-phospho-alpha-D-ribose 1-diphosphate | with guanine as cosubstrate, mutant enzyme R76S, at pH 7.5 and 25°C | Legionella pneumophila | |
0.135 | - |
guanine | with guanine as cosubstrate, mutant enzyme R76S, at pH 7.5 and 25°C | Legionella pneumophila |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GMP + diphosphate | Legionella pneumophila | - |
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | Legionella pneumophila | - |
GMP + diphosphate | - |
r | |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Legionella pneumophila | - |
IMP + diphosphate | - |
r | |
IMP + diphosphate | Legionella pneumophila | - |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Legionella pneumophila | Q5ZVC1 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 200 gel filtration | Legionella pneumophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GMP + diphosphate | - |
Legionella pneumophila | guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Legionella pneumophila | GMP + diphosphate | - |
r | |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Legionella pneumophila | IMP + diphosphate | - |
r | |
IMP + diphosphate | - |
Legionella pneumophila | hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
HGPRT | - |
Legionella pneumophila |
hypoxanthine-guanine phosphoribosyltransferase | - |
Legionella pneumophila |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
34 | - |
5-phospho-alpha-D-ribose 1-diphosphate | with guanine as cosubstrate, mutant enzyme R76S, at pH 7.5 and 25°C | Legionella pneumophila | |
53 | - |
guanine | with guanine as cosubstrate, mutant enzyme R76S, at pH 7.5 and 25°C | Legionella pneumophila | |
1200 | - |
5-phospho-alpha-D-ribose 1-diphosphate | with guanine as cosubstrate, wild type enzyme, at pH 7.5 and 25°C | Legionella pneumophila | |
5100 | - |
guanine | with guanine as cosubstrate, wild type enzyme, at pH 7.5 and 25°C | Legionella pneumophila |