Protein Variants | Comment | Organism |
---|---|---|
E196A | site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme | Homo sapiens |
E196D | site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme | Homo sapiens |
E196Q | site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme | Homo sapiens |
E196R | site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme | Homo sapiens |
E196V | a naturally occuring mutation that leads to the Lesch-Nyhan syndrome | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics | Homo sapiens | |
0.0024 | - |
IMP | wild-type enzyme | Homo sapiens | |
0.0028 | - |
hypoxanthine | mutant E196R | Homo sapiens | |
0.0036 | - |
hypoxanthine | mutant E196Q | Homo sapiens | |
0.0038 | - |
hypoxanthine | wild-type enzyme | Homo sapiens | |
0.0049 | - |
hypoxanthine | mutant E196D | Homo sapiens | |
0.0063 | - |
hypoxanthine | mutant E196A | Homo sapiens | |
0.0147 | - |
IMP | mutant E196Q | Homo sapiens | |
0.0175 | - |
diphosphate | wild-type enzyme | Homo sapiens | |
0.0191 | - |
5-phospho-alpha-D-ribose 1-diphosphate | wild-type enzyme | Homo sapiens | |
0.0726 | - |
IMP | mutant E196D | Homo sapiens | |
0.185 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant E196D | Homo sapiens | |
0.2 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant E196Q | Homo sapiens | |
0.659 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant E196R | Homo sapiens | |
0.968 | - |
diphosphate | mutant E196D | Homo sapiens | |
1.043 | - |
IMP | mutant E196A | Homo sapiens | |
1.156 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant E196A | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Homo sapiens | - |
IMP + diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | binding structure involving residue K68 at the AB-dimer interface and residues V96 and D97 of the opposing subunit, overview. Residues E133 and D137 block the binding of 5-phospho-alpha-D-ribose 1-diphosphate, mechanism, overview | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Homo sapiens | IMP + diphosphate | - |
r | |
additional information | substrate binding structure involving residue K68 at the AB-dimer interface and residues V96 and D97 of the opposing subunit, overview. Residues E133 and D137 block the binding of 5-phospho-alpha-D-ribose 1-diphosphate, mechanism, overview | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HPRT | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.038 | - |
IMP | wild-type enzyme | Homo sapiens | |
0.041 | - |
diphosphate | wild-type enzyme | Homo sapiens | |
0.18 | - |
IMP | mutant E196D | Homo sapiens | |
0.23 | - |
IMP | mutant E196A | Homo sapiens | |
0.33 | - |
diphosphate | mutant E196D | Homo sapiens | |
0.51 | - |
IMP | mutant E196Q | Homo sapiens | |
2.5 | - |
5-phospho-alpha-D-ribose 1-diphosphate | wild-type enzyme | Homo sapiens | |
2.6 | - |
hypoxanthine | wild-type enzyme | Homo sapiens | |
2.8 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant E196R | Homo sapiens | |
3.8 | - |
hypoxanthine | mutant E196R | Homo sapiens | |
10.9 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant E196Q | Homo sapiens | |
13.7 | - |
hypoxanthine | mutant E196Q | Homo sapiens | |
13.8 | - |
hypoxanthine | mutant E196D | Homo sapiens | |
14.2 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant E196D | Homo sapiens | |
20.3 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant E196A | Homo sapiens | |
20.3 | - |
hypoxanthine | mutant E196A | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
diphosphate | mutant E196D | Homo sapiens | |
0.002 | - |
diphosphate | wild-type enzyme | Homo sapiens | |
0.0024 | - |
IMP | mutant E196D | Homo sapiens | |
0.004 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant E196R | Homo sapiens | |
0.016 | - |
IMP | wild-type enzyme | Homo sapiens | |
0.02 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant E196A | Homo sapiens | |
0.034 | - |
IMP | mutant E196Q | Homo sapiens | |
0.05 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant E196Q | Homo sapiens | |
0.08 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant E196D | Homo sapiens | |
0.1 | - |
5-phospho-alpha-D-ribose 1-diphosphate | wild-type enzyme | Homo sapiens | |
0.7 | - |
hypoxanthine | wild-type enzyme | Homo sapiens | |
1 | - |
IMP | mutant E196A | Homo sapiens | |
1.4 | - |
hypoxanthine | mutant E196R | Homo sapiens | |
2.8 | - |
hypoxanthine | mutant E196D | Homo sapiens | |
3.2 | - |
hypoxanthine | mutant E196A | Homo sapiens | |
3.8 | - |
hypoxanthine | mutant E196Q | Homo sapiens |