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Literature summary for 2.4.2.8 extracted from

  • Canyuk, B.; E-Wan, A.; Keawwijit, W.; Nualnoi, T.; Sirisatean, L.; Tansakul, P.; Tanthana, C.
    The role for glutamic acid at position 196 in human hypoxanthine phosphoribosyltransferase (HPRT) as investigated using site-directed mutagenesis (2008), Nucleosides Nucleotides Nucleic Acids, 27, 894-899.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
E196A site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme Homo sapiens
E196D site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme Homo sapiens
E196Q site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme Homo sapiens
E196R site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme Homo sapiens
E196V a naturally occuring mutation that leads to the Lesch-Nyhan syndrome Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state kinetics Homo sapiens
0.0024
-
IMP wild-type enzyme Homo sapiens
0.0028
-
hypoxanthine mutant E196R Homo sapiens
0.0036
-
hypoxanthine mutant E196Q Homo sapiens
0.0038
-
hypoxanthine wild-type enzyme Homo sapiens
0.0049
-
hypoxanthine mutant E196D Homo sapiens
0.0063
-
hypoxanthine mutant E196A Homo sapiens
0.0147
-
IMP mutant E196Q Homo sapiens
0.0175
-
diphosphate wild-type enzyme Homo sapiens
0.0191
-
5-phospho-alpha-D-ribose 1-diphosphate wild-type enzyme Homo sapiens
0.0726
-
IMP mutant E196D Homo sapiens
0.185
-
5-phospho-alpha-D-ribose 1-diphosphate mutant E196D Homo sapiens
0.2
-
5-phospho-alpha-D-ribose 1-diphosphate mutant E196Q Homo sapiens
0.659
-
5-phospho-alpha-D-ribose 1-diphosphate mutant E196R Homo sapiens
0.968
-
diphosphate mutant E196D Homo sapiens
1.043
-
IMP mutant E196A Homo sapiens
1.156
-
5-phospho-alpha-D-ribose 1-diphosphate mutant E196A Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate Homo sapiens
-
IMP + diphosphate
-
r

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Reaction

Reaction Comment Organism Reaction ID
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate binding structure involving residue K68 at the AB-dimer interface and residues V96 and D97 of the opposing subunit, overview. Residues E133 and D137 block the binding of 5-phospho-alpha-D-ribose 1-diphosphate, mechanism, overview Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Homo sapiens IMP + diphosphate
-
r
additional information substrate binding structure involving residue K68 at the AB-dimer interface and residues V96 and D97 of the opposing subunit, overview. Residues E133 and D137 block the binding of 5-phospho-alpha-D-ribose 1-diphosphate, mechanism, overview Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
HPRT
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.038
-
IMP wild-type enzyme Homo sapiens
0.041
-
diphosphate wild-type enzyme Homo sapiens
0.18
-
IMP mutant E196D Homo sapiens
0.23
-
IMP mutant E196A Homo sapiens
0.33
-
diphosphate mutant E196D Homo sapiens
0.51
-
IMP mutant E196Q Homo sapiens
2.5
-
5-phospho-alpha-D-ribose 1-diphosphate wild-type enzyme Homo sapiens
2.6
-
hypoxanthine wild-type enzyme Homo sapiens
2.8
-
5-phospho-alpha-D-ribose 1-diphosphate mutant E196R Homo sapiens
3.8
-
hypoxanthine mutant E196R Homo sapiens
10.9
-
5-phospho-alpha-D-ribose 1-diphosphate mutant E196Q Homo sapiens
13.7
-
hypoxanthine mutant E196Q Homo sapiens
13.8
-
hypoxanthine mutant E196D Homo sapiens
14.2
-
5-phospho-alpha-D-ribose 1-diphosphate mutant E196D Homo sapiens
20.3
-
5-phospho-alpha-D-ribose 1-diphosphate mutant E196A Homo sapiens
20.3
-
hypoxanthine mutant E196A Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0003
-
diphosphate mutant E196D Homo sapiens
0.002
-
diphosphate wild-type enzyme Homo sapiens
0.0024
-
IMP mutant E196D Homo sapiens
0.004
-
5-phospho-alpha-D-ribose 1-diphosphate mutant E196R Homo sapiens
0.016
-
IMP wild-type enzyme Homo sapiens
0.02
-
5-phospho-alpha-D-ribose 1-diphosphate mutant E196A Homo sapiens
0.034
-
IMP mutant E196Q Homo sapiens
0.05
-
5-phospho-alpha-D-ribose 1-diphosphate mutant E196Q Homo sapiens
0.08
-
5-phospho-alpha-D-ribose 1-diphosphate mutant E196D Homo sapiens
0.1
-
5-phospho-alpha-D-ribose 1-diphosphate wild-type enzyme Homo sapiens
0.7
-
hypoxanthine wild-type enzyme Homo sapiens
1
-
IMP mutant E196A Homo sapiens
1.4
-
hypoxanthine mutant E196R Homo sapiens
2.8
-
hypoxanthine mutant E196D Homo sapiens
3.2
-
hypoxanthine mutant E196A Homo sapiens
3.8
-
hypoxanthine mutant E196Q Homo sapiens