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Literature summary for 2.4.2.8 extracted from
- Cloning, purification, and characterization of thermostable hypoxanthine-guanine phosphoribosyltransferase from Thermoanaerobacter tengcongensis (2003), Protein Expr. Purif., 32, 239-245.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) as soluble protein | Caldanaerobacter subterraneus subsp. tengcongensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0024 | - |
hypoxanthine | pH 7.4, 25°C | Caldanaerobacter subterraneus subsp. tengcongensis |  |
| 0.0027 | - |
hypoxanthine | pH 7.4, 50°C | Caldanaerobacter subterraneus subsp. tengcongensis | |
| 0.0032 | - |
guanine | pH 7.4, 50°C | Caldanaerobacter subterraneus subsp. tengcongensis | |
| 0.0033 | - |
hypoxanthine | pH 7.4, 37°C | Caldanaerobacter subterraneus subsp. tengcongensis | |
| 0.0036 | - |
guanine | pH 7.4, 25°C | Caldanaerobacter subterraneus subsp. tengcongensis | |
| 0.0042 | - |
guanine | pH 7.4, 37°C | Caldanaerobacter subterraneus subsp. tengcongensis | |
| 0.112 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 50°C, with guanine | Caldanaerobacter subterraneus subsp. tengcongensis | |
| 0.116 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 37°C, with guanine | Caldanaerobacter subterraneus subsp. tengcongensis | |
| 0.119 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 37°C, with hypoxanthine | Caldanaerobacter subterraneus subsp. tengcongensis | |
| 0.123 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 25°C, with hypoxanthine | Caldanaerobacter subterraneus subsp. tengcongensis | |
| 0.125 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 25°C, with guanine | Caldanaerobacter subterraneus subsp. tengcongensis | |
| 0.138 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 50°C, with hypoxanthine | Caldanaerobacter subterraneus subsp. tengcongensis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Caldanaerobacter subterraneus subsp. tengcongensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22800 | - |
4 * 22800, SDS-PAGE | Caldanaerobacter subterraneus subsp. tengcongensis |
| 87400 | - |
recombinant enzyme, gel filtration | Caldanaerobacter subterraneus subsp. tengcongensis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Caldanaerobacter subterraneus subsp. tengcongensis | 6-oxopurine salvage pathway reaction | IMP + diphosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caldanaerobacter subterraneus subsp. tengcongensis | Q8R7L0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli, 16fold to homogeneity by ion exchange and adsorption chromatography, and gel filtration | Caldanaerobacter subterraneus subsp. tengcongensis |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | reaction mechanism | Caldanaerobacter subterraneus subsp. tengcongensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Caldanaerobacter subterraneus subsp. tengcongensis |
| 1.8 | - |
purified recombinant enzyme | Caldanaerobacter subterraneus subsp. tengcongensis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, purified recombinant enzyme at 0.5 mg/ml, 100 mM Tris-HCl, pH 7.4, 12 mM MgCl2, 3 months, no loss of activity | Caldanaerobacter subterraneus subsp. tengcongensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Caldanaerobacter subterraneus subsp. tengcongensis | GMP + diphosphate | - |
r | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | 6-oxopurine salvage pathway reaction | Caldanaerobacter subterraneus subsp. tengcongensis | IMP + diphosphate | - |
r | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | hypoxanthine is the preferred substrate | Caldanaerobacter subterraneus subsp. tengcongensis | IMP + diphosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 22800, SDS-PAGE | Caldanaerobacter subterraneus subsp. tengcongensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HGPRT | - |
Caldanaerobacter subterraneus subsp. tengcongensis |
| hypoxanthine-guanine phosphoribosyltransferase | - |
Caldanaerobacter subterraneus subsp. tengcongensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
forward reaction | Caldanaerobacter subterraneus subsp. tengcongensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
half-life: 75 min, irreversible inactivation | Caldanaerobacter subterraneus subsp. tengcongensis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0024 | - |
guanine | pH 7.4, 25°C | Caldanaerobacter subterraneus subsp. tengcongensis | |
| 0.0027 | - |
guanine | pH 7.4, 50°C | Caldanaerobacter subterraneus subsp. tengcongensis | |
| 0.0033 | - |
guanine | pH 7.4, 37°C | Caldanaerobacter subterraneus subsp. tengcongensis | |
| 4.12 | - |
hypoxanthine | pH 7.4, 25°C | Caldanaerobacter subterraneus subsp. tengcongensis | |
| 7.97 | - |
hypoxanthine | pH 7.4, 37°C | Caldanaerobacter subterraneus subsp. tengcongensis | |
| 15.9 | - |
hypoxanthine | pH 7.4, 50°C | Caldanaerobacter subterraneus subsp. tengcongensis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
forward reaction | Caldanaerobacter subterraneus subsp. tengcongensis |
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