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Literature summary for 2.4.2.8 extracted from
- Nucleotide synthesis from 5-phosphoribosylpyrophosphate (1963), Methods Enzymol., 6, 136-158.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
hypoxanthine | with 6-mercaptopurine | Bos taurus |  |
| 0.5 | - |
guanine | - |
Bos taurus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Bos taurus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.39 | - |
purified enzyme, reverse reaction | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-mercaptopurine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Bos taurus | 6-mercaptopurine ribotide + diphosphate | - |
? | |
| guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Bos taurus | GMP + diphosphate | - |
r | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | equilibrium lies far in direction of IMP formation | Bos taurus | IMP + diphosphate | - |
r | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | 2 assay methods | Bos taurus | IMP + diphosphate | - |
r | |
| additional information | no substrates: 5-formamido-4-imidazolecarboxamide, uric acid, 8-azaguanine, 2,6-diaminopurine, orotic acid, phosphate | Bos taurus | ? | - |
? | |
| additional information | no substrate: adenine | Bos taurus | ? | - |
? | |
| xanthine + 5-phospho-alpha-D-ribose 1-diphosphate | no activity | Bos taurus | XMP + diphosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 38 | - |
assay at | Bos taurus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 65 | stable | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Bos taurus |
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Release 2023.1 (January 2023)
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