Literature summary for 2.4.2.7 extracted from

Fateev, I.V.; Sinitsina, E.V.; Bikanasova, A.U.; Kostromina, M.A.; Tuzova, E.S.; Esipova, L.V.; Muravyova, T.I.; Kayushin, A.L.; Konstantinova, I.D.; Esipov, R.S.
Thermophilic phosphoribosyltransferases Thermus thermophilus HB27 in nucleotide synthesis (2018), Beilstein J. Org. Chem., 14, 3098-3105 .

Search for more literature for 2.4.2.7

Application

Application	Comment	Organism
synthesis	purine nucleotide synthesis using TthAPRT and TthHPRT (EC 2.4.2.8)	Thermus thermophilus

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of APRT in Escherichia coli strain BL21(DE3)/pER	Thermus thermophilus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	the presence of magnesium ions promotes the proper spatial orientation of the substrate	Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
AMP + diphosphate	Thermus thermophilus	-	adenine + 5-phospho-alpha-D-ribose 1-diphosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Thermus thermophilus	Q72I82	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant APRT from Escherichia coli strain BL21(DE3)/pER by hydrophobic interaction chromatography and ultrafiltration	Thermus thermophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2,6-diaminopurine 5-phosphoribosyl nucleotide + diphosphate	low activity	Thermus thermophilus	2,6-diaminopurine + 5-phospho-alpha-D-ribose 1-diphosphate	-	r
2-aminobenzimidazole 5'-phosphoribosyl nucleotide + diphosphate	very low activity	Thermus thermophilus	2-aminobenzimidazole + 5-phospho-alpha-D-ribose 1-diphosphate	-	r
2-chloro-AMP + diphosphate	best substrate	Thermus thermophilus	2-chloroadenine + 5-phospho-alpha-D-ribose 1-diphosphate	-	r
2-fluoro-AMP + diphosphate	-	Thermus thermophilus	2-fluoroadenine + 5-phospho-alpha-D-ribose 1-diphosphate	-	r
2-methoxyadenosine 5'-phosphate + diphosphate	-	Thermus thermophilus	2-methoxyadenine + 5-phospho-alpha-D-ribose 1-diphosphate	-	r
allopurinol + 5-phospho-alpha-D-ribose 1-diphosphate	-	Thermus thermophilus	?	-	r
AMP + diphosphate	-	Thermus thermophilus	adenine + 5-phospho-alpha-D-ribose 1-diphosphate	-	r
additional information	purine nucleotide synthesis using TthAPRT and TthHPRT (EC 2.4.2.8). No or poor activity with 8-azaguanine, thymine, 1,2,4-triazole-3-carboxamide, and 1,2,4-triazole-3-carboxy-N-methylamide	Thermus thermophilus	?	-	-
N1-methyladenosine 5'-phosphate + diphosphate	high activity	Thermus thermophilus	N1-methyladenosine + 5-phospho-alpha-D-ribose 1-diphosphate	-	r
N6-benzyladenosine 5'-phosphate + diphosphate	very low activity	Thermus thermophilus	N6-benzyladenine + 5-phospho-alpha-D-ribose 1-diphosphate	-	r

Synonyms

Synonyms	Comment	Organism
TthAPRT	-	Thermus thermophilus
TT_C1250	-	Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
75	-	-	Thermus thermophilus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Thermus thermophilus

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Release 2023.1 (January 2023)