Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
G168E | the mutant of isoform APT1 shows reduced activity with zeatin (about 14%) and adenine (about 28%), respectively, compared to the wild type enzyme | Arabidopsis thaliana |
G195D | the mutant of isoform APT1 shows nearly no activity with zeatin and adenine, respectively, compared to the wild type enzyme | Arabidopsis thaliana |
G196R | the mutant of isoform APT1 shows nearly no activity with zeatin and adenine, respectively, compared to the wild type enzyme | Arabidopsis thaliana |
L96F | the mutant of isoform APT1 shows reduced activity with zeatin (about 11%) and adenine (about 8%), respectively, compared to the wild type enzyme | Arabidopsis thaliana |
P85T | the mutant of isoform APT1 shows nearly no activity with zeatin and adenine, respectively, compared to the wild type enzyme | Arabidopsis thaliana |
R149K | the mutant of isoform APT1 shows no activity with zeatin and adenine, respectively, compared to the wild type enzyme | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | P31166 | ecotype Col-0 | - |
Arabidopsis thaliana | Q42563 | ecotype Col-0 | - |
Arabidopsis thaliana | Q9LFP0 | ecotype Col-0 | - |
Arabidopsis thaliana | Q9SU38 | ecotype Col-0 | - |
Purification (Comment) | Organism |
---|---|
Ni affinity column chromatography | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenine + 5-phospho-alpha-D-ribose 1-diphosphate | isoform APT1 shows 100% activity with adenine | Arabidopsis thaliana | AMP + diphosphate | - |
? | |
additional information | isoform APT2 is nearly inactive with adenine and zeatin | Arabidopsis thaliana | ? | - |
? | |
additional information | isoform APT4 is nearly inactive with adenine | Arabidopsis thaliana | ? | - |
? | |
additional information | isoform APT5 is nearly inactive with adenine | Arabidopsis thaliana | ? | - |
? | |
zeatin + 5-phospho-alpha-D-ribose 1-diphosphate | isoform APT4 shows about 38% activity with zeatin | Arabidopsis thaliana | zeatin riboside 5'-phosphate + diphosphate | - |
? | |
zeatin + 5-phospho-alpha-D-ribose 1-diphosphate | isoform APT5 shows 100% activity with zeatin | Arabidopsis thaliana | zeatin riboside 5'-phosphate + diphosphate | - |
? | |
zeatin + 5-phospho-alpha-D-ribose 1-diphosphate | isoforms APT1 shows 100% activity with zeatin | Arabidopsis thaliana | zeatin riboside 5'-phosphate + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
adenine phosphoribosyl transferase | - |
Arabidopsis thaliana |
APRT | - |
Arabidopsis thaliana |
APT1 | isoform | Arabidopsis thaliana |
APT2 | isoform | Arabidopsis thaliana |
APT4 | isoform | Arabidopsis thaliana |
APT5 | isoform | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | loss of enzyme activity leads to excess accumulation of cytokinin bases, thus evoking myriad cytokinin-regulated responses, such as delayed leaf senescence, anthocyanin accumulation, and downstream gene expression | Arabidopsis thaliana |
metabolism | adenine phosphoribosyl transferase 1 is a key metabolic enzyme participating in the cytokinin inactivation by phosphoribosylation | Arabidopsis thaliana |