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Literature summary for 2.4.2.7 extracted from
- Specificities and pH profiles of adenine and hypoxanthine-guanine-xanthine phosphoribosyltransferases (nucleotide synthases) of the thermoacidophile archaeon Sulfolobus solfataricus (2014), Extremophiles, 18, 179-187.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Saccharolobus solfataricus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D99N | mutant enzyme has very low activity | Saccharolobus solfataricus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | is a much stronger inhibitor than ATP at pH 4.5, but has virtually no effect on activity at neutral pH | Saccharolobus solfataricus |  |
| AMP | - |
Saccharolobus solfataricus | |
| ATP | - |
Saccharolobus solfataricus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24200 | - |
2 * 24200, SDS-PAGE | Saccharolobus solfataricus |
| 45000 | - |
gel filtration | Saccharolobus solfataricus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | - |
- |
- |
| Saccharolobus solfataricus P2 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adenine + 5-phospho-alpha-D-ribose 1-diphosphate | strong preference for adenine as substrate over the 6-oxopurine bases, hypoxanthine and guanine | Saccharolobus solfataricus | AMP + diphosphate | - |
? | |
| adenine + 5-phospho-alpha-D-ribose 1-diphosphate | strong preference for adenine as substrate over the 6-oxopurine bases, hypoxanthine and guanine | Saccharolobus solfataricus P2 | AMP + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 24200, SDS-PAGE | Saccharolobus solfataricus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| adenione PRTase | - |
Saccharolobus solfataricus |
| APRTase | - |
Saccharolobus solfataricus |
| apT | - |
Saccharolobus solfataricus |
| gpT-1 | - |
Saccharolobus solfataricus |
| SSO2342 | - |
Saccharolobus solfataricus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
the enzyme shows two pH optima, i.e., around 8 and 4.5. The activity of APRTase is considerably higher at pH 8 than pH 4.5, when assayed at 35°C, while the two activity peaks seems more equal, when assayed at 60°C | Saccharolobus solfataricus |
| 7.5 | 8.5 | the enzyme shows two pH optima, i.e., around 8 and 4.5. The activity of APRTase is considerably higher at pH 8 than pH 4.5, when assayed at 35°C, while the two activity peaks seems more equal, when assayed at 60°C | Saccharolobus solfataricus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.019 | - |
ADP | 60°C, pH 4.1 | Saccharolobus solfataricus | |
| 0.177 | - |
AMP | 60°C, pH 4.1 | Saccharolobus solfataricus | |
| 0.3 | - |
AMP | 60°C, pH 7.5 | Saccharolobus solfataricus | |
| 0.344 | - |
ATP | 60°C, pH 4.1 | Saccharolobus solfataricus | |
| 0.574 | - |
ATP | 60°C, pH 7.5 | Saccharolobus solfataricus | |
| 3.2 | - |
ADP | 60°C, pH 7.5 | Saccharolobus solfataricus | |
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