Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Leishmania donovani |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | - |
Leishmania donovani | |
diphosphate | competitive against adenine and 5-phosphoribose 1-diphosphate | Leishmania donovani | |
formycin AMP | competitive against adenine and diphosphate | Leishmania donovani | |
trinitrophenyl-AMP | - |
Leishmania donovani |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Leishmania donovani | |
0.00233 | - |
adenine | - |
Leishmania donovani | |
0.00523 | - |
AMP | - |
Leishmania donovani | |
0.025 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Leishmania donovani | |
0.255 | - |
diphosphate | - |
Leishmania donovani |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania donovani | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli | Leishmania donovani |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate | ordered bi-bi mechanism, kinetics | Leishmania donovani |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Leishmania donovani | AMP + diphosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Leishmania donovani |
More | three-dimensional structure, dimeric | Leishmania donovani |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
27 | - |
recombinant enzyme, assay at | Leishmania donovani |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11.3 | - |
diphosphate | - |
Leishmania donovani | |
17.9 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Leishmania donovani |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
recombinant enzyme, assay at | Leishmania donovani |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.039 | - |
AMP | - |
Leishmania donovani | |
0.059 | - |
formycin AMP | competitive versus AMP | Leishmania donovani | |
0.062 | - |
formycin AMP | competitive versus diphosphate | Leishmania donovani | |
0.51 | - |
diphosphate | competitive versus 5-phosphoribose 1-diphosphate | Leishmania donovani | |
0.53 | - |
diphosphate | competitive versus adenine | Leishmania donovani |