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Literature summary for 2.4.2.7 extracted from

  • Bashor, C.; Denu, J.M.; Brennan, R.G.; Ullman, B.
    Kinetic mechanism of adenine phosphoribosyltransferase from Leishmania donovani (2002), Biochemistry, 41, 4020-4031.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Leishmania donovani

Inhibitors

Inhibitors Comment Organism Structure
AMP
-
Leishmania donovani
diphosphate competitive against adenine and 5-phosphoribose 1-diphosphate Leishmania donovani
formycin AMP competitive against adenine and diphosphate Leishmania donovani
trinitrophenyl-AMP
-
Leishmania donovani

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Leishmania donovani
0.00233
-
adenine
-
Leishmania donovani
0.00523
-
AMP
-
Leishmania donovani
0.025
-
5-phospho-alpha-D-ribose 1-diphosphate
-
Leishmania donovani
0.255
-
diphosphate
-
Leishmania donovani

Organism

Organism UniProt Comment Textmining
Leishmania donovani
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant from Escherichia coli Leishmania donovani

Reaction

Reaction Comment Organism Reaction ID
AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate ordered bi-bi mechanism, kinetics Leishmania donovani

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Leishmania donovani AMP + diphosphate
-
r

Subunits

Subunits Comment Organism
dimer
-
Leishmania donovani
More three-dimensional structure, dimeric Leishmania donovani

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
27
-
recombinant enzyme, assay at Leishmania donovani

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11.3
-
diphosphate
-
Leishmania donovani
17.9
-
5-phospho-alpha-D-ribose 1-diphosphate
-
Leishmania donovani

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
recombinant enzyme, assay at Leishmania donovani

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.039
-
AMP
-
Leishmania donovani
0.059
-
formycin AMP competitive versus AMP Leishmania donovani
0.062
-
formycin AMP competitive versus diphosphate Leishmania donovani
0.51
-
diphosphate competitive versus 5-phosphoribose 1-diphosphate Leishmania donovani
0.53
-
diphosphate competitive versus adenine Leishmania donovani