Any feedback?
Literature summary for 2.4.2.7 extracted from
- Purification and properties of rat liver adenine phosphoribosyltransferase (1975), Biochim. Biophys. Acta, 384, 87-101.
General Stability
| General Stability | Organism |
|---|---|
| dialysis against 20 mM Tris-HCl, pH 7.5, 20 mM (NH4)2SO4 + 5 mM 2-mercaptoethanol inactivates | Rattus norvegicus |
| Mg2+ stabilizes | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | reversible by 2-mercaptoethanol and excess Mg2+ | Rattus norvegicus |  |
| Hg2+ | reversed by 2-mercaptoethanol | Rattus norvegicus | |
| p-chloromercuribenzoate | reversed by 2-mercaptoethanol and excess Mg2+ | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Rattus norvegicus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | not required | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17500 | - |
x * 17500, SDS-PAGE | Rattus norvegicus |
| 22000 | - |
sucrose density gradient sedimentation | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.1 | - |
purified enzyme | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 2-4°C, purified enzyme in the ampholine gradient solution, half-life of 2-4 weeks, dilution inactivates | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adenine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Rattus norvegicus | AMP + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 17500, SDS-PAGE | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 5.65 | Rattus norvegicus |
| 10 | - |
- |
Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 10 | active over a broad range increasing progressively in activity from pH 5.5-10 | Rattus norvegicus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
