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Literature summary for 2.4.2.60 extracted from

  • Joshi, J.; Beaudoin, G.; Patterson, J.; García-García, J.; Belisle, C.; Chang, L.; Li, L.; Duncan, O.; Millar, A.; Hanson, A.
    Bioinformatic and experimental evidence for suicidal and catalytic plant THI4s (2020), Biochem. J., 477, 2055-2069 .
    View publication on PubMed
Search for more literature for 2.4.2.60

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) CodonPlus cells Triticum aestivum
expressed in Escherichia coli BL21(DE3) CodonPlus cells Hordeum vulgare
expressed in Escherichia coli BL21(DE3) CodonPlus cells Arabidopsis thaliana
expressed in Escherichia coli BL21(DE3) CodonPlus cells Avena sativa

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ dependent on Triticum aestivum
Fe2+ dependent on Hordeum vulgare
Fe2+ dependent on Arabidopsis thaliana
Fe2+ dependent on Avena sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine Triticum aestivum
-
 nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O
-
 ?
NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine Hordeum vulgare
-
 nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O
-
 ?
NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine Arabidopsis thaliana
-
 nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O
-
 ?
NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine Avena sativa
-
 nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
 ecotype Col-0
-
Avena sativa
-
-
-
Hordeum vulgare
-
 cultivar Morex
-
Triticum aestivum
-
 cultivars Wyalkatchem and Chinese Spring
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+ affinity column chromatography Triticum aestivum
Ni2+ affinity column chromatography Hordeum vulgare
Ni2+ affinity column chromatography Arabidopsis thaliana
Ni2+ affinity column chromatography Avena sativa

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Triticum aestivum
-
leaf
-
 Hordeum vulgare
-
leaf
-
 Arabidopsis thaliana
-
leaf
-
 Avena sativa
-
root
-
 Triticum aestivum
-
root
-
 Hordeum vulgare
-
root
-
 Arabidopsis thaliana
-
root
-
 Avena sativa
-
seed enzyme without active-site Cys is the major enzyme type in developing grains Triticum aestivum
-
seed enzyme without active-site Cys is the major enzyme type in developing grains Hordeum vulgare
-
seed enzyme without active-site Cys is the major enzyme type in developing grains Arabidopsis thaliana
-
seed enzyme without active-site Cys is the major enzyme type in developing grains Avena sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine
-
 Triticum aestivum nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O
-
 ?
NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine
-
 Hordeum vulgare nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O
-
 ?
NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine
-
 Arabidopsis thaliana nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O
-
 ?
NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine
-
 Avena sativa nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O
-
 ?

Synonyms

Synonyms Comment Organism
Thi4
-
 Triticum aestivum
Thi4
-
 Hordeum vulgare
Thi4
-
 Arabidopsis thaliana
Thi4
-
 Avena sativa

General Information

General Information Comment Organism
metabolism the enzyme synthesizes the thiazole precursor of thiamin Triticum aestivum
metabolism the enzyme synthesizes the thiazole precursor of thiamin Hordeum vulgare
metabolism the enzyme synthesizes the thiazole precursor of thiamin Arabidopsis thaliana
metabolism the enzyme synthesizes the thiazole precursor of thiamin Avena sativa
physiological function enzyme without active-site Cys is the major enzyme type in developing grains that saves substantial energy during the grain-filling period Triticum aestivum
physiological function enzyme without active-site Cys is the major enzyme type in developing grains that saves substantial energy during the grain-filling period Hordeum vulgare
physiological function enzyme without active-site Cys is the major enzyme type in developing grains that saves substantial energy during the grain-filling period Arabidopsis thaliana
physiological function enzyme without active-site Cys is the major enzyme type in developing grains that saves substantial energy during the grain-filling period Avena sativa