Cloned (Comment) | Organism |
---|---|
gene pdt, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Trypanosoma brucei brucei |
Protein Variants | Comment | Organism |
---|---|---|
N53D | site-directed mutagenesis, the mutant shows no ribosyltransferase activity and reduced activity compared to the wild-type enzyme | Trypanosoma brucei brucei |
Y5F | site-directed mutagenesis, the deoxyribose/ribose selectivity ratio improves about tenfold compared to wild-type if Tyr5 is replaced with a phenylalanine, the mutant shows reduced activity compared to the wild-type enzyme | Trypanosoma brucei brucei |
Y5F/N53D | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Trypanosoma brucei brucei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-D-ribosyl-base1 + base2 | Trypanosoma brucei brucei | - |
2-deoxy-D-ribosyl-base2 + base1 | - |
? | |
2-deoxy-D-ribosyl-base1 + base2 | Trypanosoma brucei brucei 927/4 GUTat10.1 | - |
2-deoxy-D-ribosyl-base2 + base1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei brucei | Q57VC7 | - |
- |
Trypanosoma brucei brucei 927/4 GUTat10.1 | Q57VC7 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Trypanosoma brucei brucei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.1 | - |
below, recombinant mutant Y5F/N53D with substrates inosine and adenine, pH 4.0, 50°C | Trypanosoma brucei brucei |
0.1 | - |
below, recombinant wild-type enzyme with substrates inosine and adenine, pH 4.0, 50°C | Trypanosoma brucei brucei |
0.2 | - |
recombinant mutant N53D with substrates inosine and adenine, pH 6.0, 50°C | Trypanosoma brucei brucei |
0.5 | - |
recombinant mutant Y5F with substrates inosine and adenine, pH 4.0, 50°C | Trypanosoma brucei brucei |
0.9 | - |
recombinant wild-type enzyme with substrates inosine and adenine, pH 6.0, 50°C | Trypanosoma brucei brucei |
1.6 | - |
recombinant mutant Y5F/N53D with substrates inosine and adenine, pH 6.0, 50°C | Trypanosoma brucei brucei |
2.7 | - |
recombinant mutant Y5F with substrates inosine and adenine, pH 6.0, 50°C | Trypanosoma brucei brucei |
7.3 | - |
recombinant mutant Y5F/N53D with substrates 2'-deoxyinosine and adenine, pH 6.0, 50°C | Trypanosoma brucei brucei |
11 | - |
recombinant mutant N53D with substrates 2'-deoxyinosine and adenine, pH 6.0, 50°C | Trypanosoma brucei brucei |
11.1 | - |
recombinant mutant Y5F/N53D with substrates 2'-deoxyinosine and adenine, pH 4.0, 50°C | Trypanosoma brucei brucei |
11.3 | - |
recombinant mutant Y5F with substrates 2'-deoxyinosine and adenine, pH 6.0, 50°C | Trypanosoma brucei brucei |
12.3 | - |
recombinant mutant Y5F with substrates 2'-deoxyinosine and adenine, pH 4.0, 50°C | Trypanosoma brucei brucei |
38.5 | - |
recombinant wild-type enzyme with substrates 2'-deoxyinosine and adenine, pH 6.0, 50°C | Trypanosoma brucei brucei |
47.5 | - |
recombinant mutant N53D with substrates 2'-deoxyinosine and adenine, pH 4.0, 50°C | Trypanosoma brucei brucei |
47.5 | - |
recombinant wild-type enzyme with substrates 2'-deoxyinosine and adenine, pH 4.0, 50°C | Trypanosoma brucei brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-deoxyguanosine + 2,6-diaminopurine | - |
Trypanosoma brucei brucei | 2,6-diaminopurine-2'deoxyriboside + guanine | - |
? | |
2'-deoxyguanosine + 2,6-diaminopurine | - |
Trypanosoma brucei brucei 927/4 GUTat10.1 | 2,6-diaminopurine-2'deoxyriboside + guanine | - |
? | |
2'-deoxyguanosine + 2-chloroadenine | - |
Trypanosoma brucei brucei | 2-chloro-2'-deoxyadenosine + guanine | - |
? | |
2'-deoxyguanosine + 2-chloroadenine | - |
Trypanosoma brucei brucei 927/4 GUTat10.1 | 2-chloro-2'-deoxyadenosine + guanine | - |
? | |
2'-deoxyguanosine + 2-fluoroadenine | - |
Trypanosoma brucei brucei | 2-fluoro-2'-deoxyadenosine + guanine | - |
? | |
2'-deoxyguanosine + 6-chloropurine | - |
Trypanosoma brucei brucei | 6-chloropurine-2'-deoxyriboside + guanine | - |
? | |
2'-deoxyguanosine + 6-mercaptopurine | - |
Trypanosoma brucei brucei | 6-mercaptopurine-2'-deoxyriboside + guanine | - |
? | |
2'-deoxyguanosine + 6-methoxyguanine | - |
Trypanosoma brucei brucei | 6-methoxy-2'-deoxyguanosine + guanine | - |
? | |
2'-deoxyguanosine + 6-methylpurine | - |
Trypanosoma brucei brucei | 6-methylpurine-2'-deoxyriboside + guanine | - |
? | |
2'-deoxyguanosine + adenine | - |
Trypanosoma brucei brucei | 2'-deoxyadenosine + guanine | - |
? | |
2'-deoxyguanosine + benzimidazole | - |
Trypanosoma brucei brucei | benzimidazole 2'-deoxyriboside + guanine | - |
? | |
2'-deoxyguanosine + hypoxanthine | - |
Trypanosoma brucei brucei | 2'-deoxyinosine + guanine | - |
? | |
2'-deoxyinosine + adenine | - |
Trypanosoma brucei brucei | 2'-deoxyadenosine + hypoxanthine | - |
? | |
2'-deoxyinosine + adenine | - |
Trypanosoma brucei brucei 927/4 GUTat10.1 | 2'-deoxyadenosine + hypoxanthine | - |
? | |
2-deoxy-D-ribosyl-base1 + base2 | - |
Trypanosoma brucei brucei | 2-deoxy-D-ribosyl-base2 + base1 | - |
? | |
2-deoxy-D-ribosyl-base1 + base2 | - |
Trypanosoma brucei brucei 927/4 GUTat10.1 | 2-deoxy-D-ribosyl-base2 + base1 | - |
? | |
inosine + adenine | cf. EC 2.4.2.5 | Trypanosoma brucei brucei | adenosine + hypoxanthine | - |
? | |
additional information | no activity with xanthine, 7-deazaxanthine, and 7-deazahypoxanthine, substrate specificity, overview. No significant preference exists between 6-aminopurines and 6-oxopurines as base acceptors. Binding structures of inosine within the active site of wild-type TbPDT and TbPDTY5F | Trypanosoma brucei brucei | ? | - |
- |
|
additional information | no activity with xanthine, 7-deazaxanthine, and 7-deazahypoxanthine, substrate specificity, overview. No significant preference exists between 6-aminopurines and 6-oxopurines as base acceptors. Binding structures of inosine within the active site of wild-type TbPDT and TbPDTY5F | Trypanosoma brucei brucei 927/4 GUTat10.1 | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 19000, recombinant enzyme, SDS-PAGE | Trypanosoma brucei brucei |
Synonyms | Comment | Organism |
---|---|---|
nucleoside 2'-deoxyribosyltransferase | - |
Trypanosoma brucei brucei |
Tb927.5.1360 | locus name | Trypanosoma brucei brucei |
TbPDT | - |
Trypanosoma brucei brucei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Trypanosoma brucei brucei |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
69 | - |
TbPDTY5F variant is a homodimer that shows a remarkably high melting temperature of 69°C | Trypanosoma brucei brucei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Trypanosoma brucei brucei |