Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Peribacillus psychrosaccharolyticus |
General Stability | Organism |
---|---|
the enzyme is stable when immobilized on PEI-agarose but shows no activity when immobilized on glyoxyl-agarose. The best stability is obtained using dextrane oxidized at 70% (w/v) | Peribacillus psychrosaccharolyticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | activity decreases 25% in presence of 1.5 M NaCl | Peribacillus psychrosaccharolyticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Na+ | - |
Peribacillus psychrosaccharolyticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16000 | - |
6 * 16000, SDS-PAGE | Peribacillus psychrosaccharolyticus |
91400 | - |
gel filtration | Peribacillus psychrosaccharolyticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-D-ribosyl-base1 + base2 | Peribacillus psychrosaccharolyticus | - |
2-deoxy-D-ribosyl-base2 + base1 | - |
? | |
2-deoxy-D-ribosyl-base1 + base2 | Peribacillus psychrosaccharolyticus CECT 4074 | - |
2-deoxy-D-ribosyl-base2 + base1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Peribacillus psychrosaccharolyticus | - |
- |
- |
Peribacillus psychrosaccharolyticus CECT 4074 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Bio-Scale Mini UNOsphere Q column chromatography, and Superose 12 gel filtration | Peribacillus psychrosaccharolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-deoxyuridine + 5-trifluorothymine | - |
Peribacillus psychrosaccharolyticus | 5-trifluorothymidine + uracil | - |
? | |
2'-deoxyuridine + 5-trifluorothymine | - |
Peribacillus psychrosaccharolyticus CECT 4074 | 5-trifluorothymidine + uracil | - |
? | |
2'-deoxyuridine + adenine | - |
Peribacillus psychrosaccharolyticus | 2'-deoxyadenosine + uracil | - |
? | |
2'-deoxyuridine + adenine | - |
Peribacillus psychrosaccharolyticus CECT 4074 | 2'-deoxyadenosine + uracil | - |
? | |
2'-deoxyuridine + thymidine | - |
Peribacillus psychrosaccharolyticus | 2'-deoxythymidine + uracil | - |
? | |
2'-deoxyuridine + thymidine | - |
Peribacillus psychrosaccharolyticus CECT 4074 | 2'-deoxythymidine + uracil | - |
? | |
2-deoxy-D-ribosyl-base1 + base2 | - |
Peribacillus psychrosaccharolyticus | 2-deoxy-D-ribosyl-base2 + base1 | - |
? | |
2-deoxy-D-ribosyl-base1 + base2 | - |
Peribacillus psychrosaccharolyticus CECT 4074 | 2-deoxy-D-ribosyl-base2 + base1 | - |
? | |
additional information | the enzyme is not able to interchange the deoxyribosyl moiety between ribonucleosides and is free of nucleoside phosphorylases traces | Peribacillus psychrosaccharolyticus | ? | - |
? | |
additional information | the enzyme is not able to interchange the deoxyribosyl moiety between ribonucleosides and is free of nucleoside phosphorylases traces | Peribacillus psychrosaccharolyticus CECT 4074 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homohexamer | 6 * 16000, SDS-PAGE | Peribacillus psychrosaccharolyticus |
Synonyms | Comment | Organism |
---|---|---|
NDT | - |
Peribacillus psychrosaccharolyticus |
nucleoside 2'-deoxyribosyltransferase | - |
Peribacillus psychrosaccharolyticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Peribacillus psychrosaccharolyticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 90 | the enzyme is stable up to 50°C during 15 min | Peribacillus psychrosaccharolyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Peribacillus psychrosaccharolyticus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | the enzyme is stable in a pH range from 6.0 to 9.0, and retains more than 85% activity at pH 10.0 | Peribacillus psychrosaccharolyticus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Peribacillus psychrosaccharolyticus | isoelectric focusing | - |
4.6 |