Cloned (Comment) | Organism |
---|---|
- |
Lactobacillus leichmannii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11 | - |
2'-deoxycytidine | mutant F13R/D92S/N123S, pH 6.0, 37°C | Lactobacillus leichmannii | |
22 | - |
2'-deoxycytidine | mutant F13R/D92S, pH 6.0, 37°C | Lactobacillus leichmannii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactobacillus leichmannii | Q9R5V5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-deoxyadenosine + 5-fluorouracil | reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S | Lactobacillus leichmannii | 2'-deoxy-D-ribosyl-5-fluorouracil + adenine | - |
? | |
2'-deoxycytidine + 2,6-diaminopurine | reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S | Lactobacillus leichmannii | 2,6-diaminopurine 2-deoxy-D-riboside + cytosine | - |
? | |
2'-deoxycytidine + 5-bromouracil | reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S | Lactobacillus leichmannii | 2'-deoxy-5-bromouridine + cytosine | - |
? | |
2'-deoxycytidine + 6-chloropurine | reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S | Lactobacillus leichmannii | 6-chloropurine 2-deoxy-D-ribose 5-phosphate + cytosine | - |
? | |
2'-deoxycytidine + adenine | - |
Lactobacillus leichmannii | 2'-deoxyadenosine + cytosine | - |
? | |
dCMP + 2,6-dichloropurine | reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S | Lactobacillus leichmannii | 2,6-dichloropurin-9-yl-2'-deoxy-beta-D-ribose 5'-phosphate + cytosine | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
2'-deoxycytidine | mutant F13R/D92S, pH 6.0, 37°C | Lactobacillus leichmannii | |
2.1 | - |
2'-deoxycytidine | mutant F13R/D92S/N123S, pH 6.0, 37°C | Lactobacillus leichmannii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0136 | - |
2'-deoxycytidine | mutant F13R/D92S, pH 6.0, 37°C | Lactobacillus leichmannii | |
0.192 | - |
2'-deoxycytidine | mutant F13R/D92S/N123S, pH 6.0, 37°C | Lactobacillus leichmannii |